山羊F(ab')2抗兔IgG - H&L (FITC),预吸附二抗
参阅全部 IgG 二抗
Goat
Rabbit
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, WB, Dot blotmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheepmore details
Rabbit IgG whole molecule
FITC. Ex: 493nm, Em: 528nm
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
浓度
500 µg 浓度为 1 mg/ml
500 µg 浓度为 1 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.6 moles FITC per mole of Goat IgG F(ab’)2
多克隆
IgG
Abpromise™承诺保证使用ab6009于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/1000 - 1/5000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Flow Cyt | 1/500 - 1/2500. | |
| ELISA | 1/10000 - 1/50000. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. | |
| Dot blot | Use at an assay dependent concentration. |
Immunohistochemistry (Frozen sections) - Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed (ab6009)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:
Primary antibody: Rabbit anti-laminin alpha 1 (basement membrane marker), 1:400
Secondary antibody: Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab6009), 1:100
Nuclei were counterstained with DAPI
F-actin was stained with rhodamine-phalloidin, 1:100
Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 µm thickness
