山羊F(ab')2抗小鼠IgM mu chain (PE)
参阅全部 IgM 二抗
Goat
Mouse
No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). No reaction was observed against other mouse heavy or light chain proteins.
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, Dot blotmore details
Mouse IgM mu heavy chain
PE. Ex: 488nm, Em: 575nm
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
500 µl 浓度为 0.5 mg/ml
500 µl 浓度为 0.5 mg/m
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by pepsin digestion and chromatographic separation.
R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
多克隆
IgG
Abpromise™承诺保证使用ab5932于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/100 - 1/250. | |
Immunomicroscopy | Use at an assay dependent concentration. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications. | |
Flow Cyt | 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. | |
Dot blot | Use at an assay dependent concentration. |
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