Anti-FE65抗体
参阅全部 FE65 一抗
兔多克隆抗体to FE65
Rabbit
This antibody does not detect endogenous levels of Fe65.
适用于: ICC/IF, WBmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Mouse FE65 aa 352-369.
Sequence:
PQEEEKLSQRNANPGIKC
(Peptide available as ab5892)
ICC/IF: SH-SY5Y (Human neuroblastoma cell line from bone marrow). WB: U-87 MG; SH-SY5Y; NTERA-2; U-2 OS; A549; Mouse Brain; PC-12
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5668于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 2 µg/ml. | |
| WB | Use a concentration of 0.2 µg/ml. Detects a band of approximately 97 kDa. |
Entrez Gene: 322 Human
Entrez Gene: 11785 Mouse
Omim: 602709 Human
SwissProt: O00213 Human
SwissProt: Q9QXJ1 Mouse
Unigene: 372840 Human
Unigene: 38469 Mouse
Unigene: 19953 Rat
Adaptor protein FE65a2 antibody
Amyloid beta (A4) precursor protein binding family B member 1 antibody
Amyloid beta A4 precursor protein binding family B antibody
Amyloid beta A4 precursor protein binding family B member 1 antibody
Amyloid beta A4 precursor protein-binding family B member 1 antibody
Amyloid beta precursor protein binding family B member 1 antibody
APBB 1 antibody
APBB1 antibody
APBB1_HUMAN antibody
FE 65 antibody
Fe65 protein antibody
Protein Fe65 antibody
RIR antibody
stat like protein antibody
Western blot - Anti-FE65 antibody (ab5668)
All lanes : Anti-FE65 antibody (ab5668) at 2 µg/ml
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with blocking with 5 % skimmed milk
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with blocking with 5 % skimmed milk
Lane 3 : NTERA-2 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate with blocking with 5 % skimmed milk
Lane 4 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate with blocking with 5 % skimmed milk
Lane 5 : A549 (Human lung carcinoma cell line) whole cell lysate with blocking with 5 % skimmed milk
Lane 6 : Mouse Brain with blocking with 5 % skimmed milk
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate with blocking with 5 % skimmed milk
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/2500 dilution
Additional bands at: ~77 kDa. We are unsure as to the identity of these extra bands.
Detection: chemiluminescence
Western blot - Anti-FE65 antibody (ab5668)
Western blot of Fe65-affinity pulldown materials from mouse brain lysate using ab5668. Lane 1 is an immunopurified sample from a full-length Fe65 Knockout mouse. Lane 2 is an immunopurified sample from an Fe65 wild type mouse.
Immunocytochemistry/ Immunofluorescence - Anti-FE65 antibody (ab5668)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells, blocked with 1% BSA for 1 hour at room temperature. Labelling Fe65 with ab5668 at 2 µg/mL and incubated for 3 hours at room temperature and then labelled with Goat anti-Rabbit IgG secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (Blue). F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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