Anti-SNAP25抗体
参阅全部 SNAP25 一抗
兔多克隆抗体to SNAP25
Rabbit
适用于: ICC, WB, Flow Cytmore details
与反应: Mouse, Rat
预测可用于: Human, Chimpanzee, Rhesus monkey, Goldfish
Synthetic peptide corresponding to Mouse SNAP25 aa 195-206.
Sequence:
ANQRATKMLGSG
(Peptide available as ab5843)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/100. | |
| WB | (4) | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa. |
| Flow Cyt | Use 3-5µg for 106 cells. |
Entrez Gene: 458096 Chimpanzee
Entrez Gene: 6616 Human
Entrez Gene: 20614 Mouse
Entrez Gene: 574204 Rhesus monkey
Omim: 600322 Human
SwissProt: Q5R1X1 Chimpanzee
SwissProt: P36978 Goldfish
SwissProt: P60880 Human
SwissProt: P60879 Mouse
SwissProt: P60877 Rhesus monkey
Unigene: 167317 Human
Unigene: 45953 Mouse
Unigene: 107689 Rat
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Western blot - Anti-SNAP25 antibody (ab5666)
All lanes : Anti-SNAP25 antibody (ab5666)
Lane 1 : Mouse cerebellum
Lane 2 : Mouse brain
Lane 3 : Rat brain
Lane 4 : Membrane enriched extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate
Immunocytochemistry - Anti-SNAP25 antibody (ab5666)
ICC/IF image of ab5666 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry - Anti-SNAP25 antibody (ab5666)
Immunlocalization of SNAP-25 in cultured rat hippocampal cells using ab5666.
Western blot - Anti-SNAP25 antibody (ab5666)This image is courtesy of an anonymous Abreview
Anti-SNAP25 antibody (ab5666) at 1/10000 dilution + Whole tissue lysate prepared from mouse hippocampus.
Secondary
HRP conjugated goat anti-rabbit Ig. at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 25 kDawhy is the actual band size different from the predicted?
Exposure time: 20 seconds
