Anti-PDE6D抗体
参阅全部 PDE6D 一抗
兔多克隆抗体to PDE6D
Rabbit
This antibody does not detect other PDE6 isoforms.
适用于: ICC/IF, WB, ICC, IHC-Frmore details
与反应: Mouse, Sheep, Cow, Dog, Human, Pig
Synthetic peptide corresponding to Mouse PDE6D aa 1-16.
Sequence:
MSAKDERAR(D/E)ILRGFK
(Peptide available as ab41788)
ICC/IF: B3 cells, BAEC cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5665于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/10 - 1/200. | |
| WB | (1) | Use at an assay dependent concentration. |
| ICC | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
Entrez Gene: 5147 Human
Entrez Gene: 18582 Mouse
Omim: 602676 Human
SwissProt: O43924 Human
SwissProt: O55057 Mouse
Unigene: 516808 Human
Unigene: 12925 Mouse
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Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab5665)
Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5565 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab5665)
Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5665 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
