Anti-EGFR (phospho Y1086)抗体
参阅全部 EGFR 一抗
兔多克隆抗体to EGFR (phospho Y1086)
Rabbit
适用于: ICC, WBmore details
与反应: Human
Synthetic peptide corresponding to Human EGFR (phospho Y1086).
WB: A-431 cells, A549 cells. ICC: A-431 cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.3
Constituent: PBS
Immunogen affinity purified
The antibody has been negatively preadsorbed using (i) a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated epidermal growth factor receptor (EGFR), and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an EGFR-derived peptide that is phosphorylated at tyrosine 1086.
多克隆
IgG
Abpromise™承诺保证使用ab5650于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/100. | |
| WB | (1) | Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 185 kDa. |
Entrez Gene: 1956 Human
Omim: 131550 Human
SwissProt: P00533 Human
Unigene: 488293 Human
Unigene: 605083 Human
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Immunocytochemistry - Anti-EGFR (phospho Y1086) antibody (ab5650)
A-431 cells stained for EGFR (green) using ab5650 at 1/100 dilution in ICC/IF. Followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing membranous localization. Panel e represents cells treated with antagonist, Afatinib (1uM for 6hrs) followed by EGF (200 ng/mL for 10 minutes), showing no signal. Panel f shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background.
Western blot - Anti-EGFR (phospho Y1086) antibody (ab5650)
All lanes : Anti-EGFR (phospho Y1086) antibody (ab5650) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : A549 cell lysate treated for 10 minutes with 200 ng/mL of EGF
Lane 3 : A431 cell lysate
Lane 4 : A431 cell lysate treated for 10 minutes with 200 ng/mL of EGF
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/5000 dilution
Western blot - Anti-EGFR (phospho Y1086) antibody (ab5650)
All lanes : Anti-EGFR (phospho Y1086) antibody (ab5650) at 1/1000 dilution
Lane 1 : A-431 extracts
Lane 2 : A-431 treated with EGF (200 ng/mL for 10 minutes)
Lane 3 : A-431 treated with Gefitinib followed by EGF (1uM for 16 hours, 200 ng/mL for 10 minutes)
Lane 4 : A-431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200 ng/mL for 10 minutes)
Lane 5 : A549 extracts
Lane 6 : A549 treated with EGF (200 ng/mL for 10 minutes)
Lane 7 : A549 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200 ng/mL for 10 minutes)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
