Anti-KIF5B antibody

• 产品名称

  Anti-KIF5B抗体
  参阅全部 KIF5B 一抗

• 

  Western blot - Anti-KIF5B antibody (ab5629)

  All lanes : Anti-KIF5B antibody (ab5629) at 1/2000 dilution
  
  Lane 1 : Mouse brain Whole cell extract
  Lane 2 : Mouse lung Whole cell extract
  Lane 3 : Rat brain Whole cell extract
  Lane 4 : Rat liver Whole cell extract
  
  Lysates/proteins at 40 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L),HRP at 1/4000 dilution
  
  Predicted band size: 110 kDa
  
  

• 

  Western blot - Anti-KIF5B antibody (ab5629)

  All lanes : Anti-KIF5B antibody (ab5629) at 1/4000 dilution
  
  Lane 1 : Mouse Cerebellum whole cell extract
  Lane 2 : Rat Brain whole cell extract
  Lane 3 : Mouse Brain whole cell extract
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
  
  Predicted band size: 110 kDa
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)

  ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.

• 描述

  兔多克隆抗体to KIF5B

• 宿主

  Rabbit

• 特异性

  This antibody is specific for KIF5B and does not detect other kinesin isotypes.

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Synthetic peptide corresponding to Human KIF5B aa 376-396.
  Sequence:

  IDEQFDKEKANLEAFTVDKDI

  
  Database link: P33176
  (Peptide available as ab41782)

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

• 
  

• The Abpromise guarantee

• Abpromise™承诺保证使用ab5629于以下的经测试应用

• “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 应用	Ab评论	说明
  ICC/IF	(1)	1/250.
  WB		1/2000 - 1/6000. Predicted molecular weight: 110 kDa.Can be blocked with Human KIF5B peptide (ab41782).

• 数据库链接

• Entrez Gene: 3799 Human

• Entrez Gene: 16573 Mouse

• Entrez Gene: 117550 Rat

• Omim: 602809 Human

• SwissProt: P33176 Human

• SwissProt: Q61768 Mouse

• SwissProt: Q2PQA9 Rat

• Unigene: 327736 Human

• Unigene: 223744 Mouse

• Unigene: 219283 Rat

• Unigene: 4046 Rat

别名

• Conventional kinesin heavy chain antibody

• KIF 5B antibody

• KIF5B antibody

• Kinesin 1 antibody

• kinesin 1 (110-120kD) antibody

• Kinesin 1 heavy chain antibody

• Kinesin family member 5B antibody

• Kinesin heavy chain antibody

• kinesin, heavy chain, ubiquitous antibody

• Kinesin-1 heavy chain antibody

• Kinesin1 antibody

• KINH antibody

• KINH_HUMAN antibody

• KNS 1 antibody

• KNS antibody

• KNS1 antibody

• Ubiquitous kinesin heavy chain antibody

• UKHC antibody