Anti-Proteasome活化剂Subunit 4/PSME4抗体
参阅全部 Proteasome Activator Subunit 4/PSME4 一抗
兔多克隆抗体to Proteasome活化剂Subunit 4/PSME4
Rabbit
适用于: ICC/IF, WBmore details
与反应: Mouse, Human
预测可用于: Zebrafish
Synthetic peptide corresponding to Human Proteasome Activator Subunit 4/PSME4 aa 1620-1634.
Sequence:
TKLPKKRKRDPGSVG
WB: Hela, Jurkat whole cell lysate, Mouse testis tissue lysate. ICC: HeLa cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab5620于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/600. | |
| WB | 1/250 - 1/5000. Detects a band of approximately 200 kDa. |
Entrez Gene: 23198 Human
Entrez Gene: 103554 Mouse
Omim: 607705 Human
SwissProt: Q14997 Human
SwissProt: Q5SSW2 Mouse
Unigene: 413801 Human
Unigene: 481953 Mouse
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Proteasome activator complex subunit 4 antibody
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PSME 4 antibody
psme4 antibody
PSME4_HUMAN antibody
KIAA0077 antibody
PA200 antibody
Proteasome Activator 200 kDa antibody
Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/250 dilution
Lane 1 : HeLa whole cell extract
Lane 2 : Jurkat whole cell extract
Lane 3 : Mouse testis tissue extract
Lane 4 : Mouse pancreas tissue extract
Lane 5 : Rat pancreas tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution
A 200 kDa band corresponding to PSME4 was observed in HeLa, Jurkat, and mouse testis. In addition, a 140 kDa band corresponding to an isoform of PSME4 was observed across the cell lines and tissues tested.
Blocking - 5% skimmed milk
Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/2000 dilution + Mouse testis tissue lysate at 25 µg
Secondary
HRP-conjugated anti-rabbit
Developed using the ECL technique.
Observed band size: 160,200 kDawhy is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
