Anti-PDGFR alpha (phospho Y754)抗体
参阅全部 PDGFR alpha 一抗
兔多克隆抗体to PDGFR alpha (phospho Y754)
Rabbit
适用于: IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide corresponding to Human PDGFR alpha (phospho Y754).
IHC: Mouse colo, human brain tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Immunogen affinity purified
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at tyrosine 754.
多克隆
IgG
Abpromise™承诺保证使用ab5460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (4) | 1/20. |
Entrez Gene: 5156 Human
Entrez Gene: 18595 Mouse
Omim: 173490 Human
SwissProt: P16234 Human
SwissProt: P26618 Mouse
Unigene: 74615 Human
Unigene: 221403 Mouse
Unigene: 55127 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)
Paraffin embedded mouse colon tissue stained for PDGFR alpha (phospho Y754) using ab5460 (right) at 1/20 dilution compared to a negative control section without primary antibody (left), followed by HRP-conjugated secondary antibody in immunohistochemical analysis.
Heat mediated antigen retrieval was performed using 10mM sodium citrate (pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)
Paraffin embedded human brain tissue stained for PDGFR alpha (phospho Y754) usinf ab5460 (right) at 1/20 dilution compared to a negative control section without primary antibody (left), followed by HRP-conjugated secondary antibody in immunohistochemical analysis.
Heat mediated antigen retrieval was performed using 10mM sodium citrate (pH 6.0).