Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker

• 产品名称

  Anti-ATPB抗体[4.3E8.D10] - Mitochondrial Marker
  参阅全部 ATPB 一抗

• 描述

  小鼠单克隆抗体[4.3E8.D10] to ATPB - Mitochondrial Marker

• 宿主

  Mouse

• 特异性

  Detects the beta subunit of ATP synthase (ATPB) from mouse rat and human samples. This antibody is useful as a mitochondrial marker.

• 经测试应用

  适用于: WB, ICC/IF, IPmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Tissue, cells or virus corresponding to ATPB. Intact rat mitochondria.

• 阳性对照

• ICC/IF: 3T3, HEK293T, A431, rat neuronal glial cells, HeLa; IP: rat neuronal/glial cells, THP-1 cells; WB: human testis tissue lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 99% PBS, 0.1% BSA

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  4.3E8.D10

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab5432于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 506 Human

• Entrez Gene: 11947 Mouse

• Entrez Gene: 171374 Rat

• GenBank: BC016512 Human

• Omim: 102910 Human

• SwissProt: P06576 Human

• SwissProt: P56480 Mouse

• SwissProt: P10719 Rat

• Unigene: 406510 Human

• Unigene: 238973 Mouse

• Unigene: 92965 Rat

别名

• ATP 5B antibody

• ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody

• ATP synthase subunit beta mitochondrial antibody

• ATP synthase subunit beta, mitochondrial antibody

• atp5b antibody

• ATPB antibody

• ATPB_HUMAN antibody

• ATPMB antibody

• ATPSB antibody

• Epididymis secretory protein Li 271 antibody

• HEL-S-271 antibody

• Mitochondrial ATP synthase beta subunit antibody

• Mitochondrial ATP Synthase Subunit Beta antibody

• Mitochondrial ATP synthetase beta subunit antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  Immunocytochemistry analysis of ATPB using ab5432 at 5µg/mL concentration shows staining in 4% paraformaldehyde-fixed 3T3 Cells. Secondary was Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at 1/1000 dilution. ATPB (green), and nuclei with Hoechst 33342 dye (blue) is shown. Negative control has no primary antibody

• 

  Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  
  ATPB was immunoprecipitated from THP-1 whole cell lysate with 5 µL ab5432.

  Lane 1: ab5432 IP in THP-1 whole cell lysate, with HRP-conjugated goat anti-mouse IgG secondary

   

  Detection: Chemiluminescence 

• 

  Western blot - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 20 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 57 kDa
  Observed band size: 57 kDa
  
  
  Exposure time: 16 minutes
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  Immunocytochemistry/Immunofluorescent analysis of ATPB (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with ab5432 at 1:100 overnight at 4ºC in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  Immunofluorescent analysis of ATPB in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  Immunofluorescent analysis of ATPB in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• 

  Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  Immunoprecipatation of rat neuronal/glial cell extract using ab5432.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  ICC/IF image of ab5432 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

  Immunocytochemistry/Immunofluorescence analysis of rat neuronal/glial cell culture using ab5432.