Anti-CTGF抗体
参阅全部 CTGF 一抗
兔多克隆抗体to CTGF
Rabbit
This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097.
适用于: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details
与反应: Rat, Human, Pig, Recombinant fragment
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
100 µg 浓度为 0.9 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5097于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use a concentration of 1 µg/ml. | |
| IHC-Fr | (2) | Use at an assay dependent concentration. |
| WB | 1/500. Predicted molecular weight: 38 kDa. This application has only been tested on recombinant protein. | |
| IHC-P | (6) | Use at an assay dependent concentration. PubMed: 17028989 |
| ICC/IF | (1) | Use at an assay dependent concentration. |
Entrez Gene: 1490 Human
Omim: 121009 Human
SwissProt: P29279 Human
Unigene: 410037 Human
Unigene: 17145 Rat
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Immunocytochemistry - Anti-CTGF antibody (ab5097)
ab5097 staining CTGF in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5097 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-CTGF antibody (ab5097)
Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.
Not yet tested against endogenous CTGF
Secondary antibody - anti-rabbit HRP (ab6721)
Immunocytochemistry - Anti-CTGF antibody (ab5097)This image is courtesy of an Abreview submitted by Beate Thode
ab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).
