Anti-REV1抗体
参阅全部 REV1 一抗
山羊多克隆抗体to REV1
Goat
适用于: IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog
Synthetic peptide corresponding to Human REV1 aa 1200 to the C-terminus.
Database link: Q9UBZ9-1
IHC-P: Human Testis tissue.
REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
多克隆
IgG2c
Abpromise™承诺保证使用ab5088于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Unigene: 260943 Mouse
Unigene: 389103 Mouse
Entrez Gene: 51455 Human
Entrez Gene: 56210 Mouse
GenBank: NP_057400 Human
Omim: 606134 Human
SwissProt: Q9UBZ9 Human
SwissProt: Q920Q2 Mouse
Unigene: 443077 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV1 antibody (ab5088)
In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. ab5088 used at 4ug/ml following Steamed antigen retrieval with Tris/EDTA buffer pH 9 visualised using HRP staining.