Anti-FOXC2 antibody

• 产品名称

  Anti-FOXC2抗体
  参阅全部 FOXC2 一抗

• 描述

  山羊多克隆抗体to FOXC2

• 宿主

  Goat

• 经测试应用

  适用于: WB, ChIPmore details
  不适用于: IHC-Fr

• 种属反应性

  与反应: Mouse, Human, African green monkey
  预测可用于: Rat, Cow, Dog

• 免疫原

  Synthetic peptide:

  RHAAPYSYDCTKY

  , corresponding to C terminal amino acids 489-501 of Human FOXC2.

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: HEK-293 nuclear lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.02% Sodium azide
  Constituents: Tris buffered saline, 0.5% BSA

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5060于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IHC-Fr.

数据库链接

• Entrez Gene: 2303 Human

• Entrez Gene: 14234 Mouse

• Entrez Gene: 171356 Rat

• Omim: 602402 Human

• SwissProt: Q99958 Human

• SwissProt: Q61850 Mouse

• SwissProt: Q63246 Rat

• Unigene: 436448 Human

• Unigene: 14092 Mouse

• Unigene: 216723 Rat

别名

• Drosphilia Forkhead Homolog Like 14 antibody

• FKHL 14 antibody

• FKHL14 antibody

• Forkhead Box C2 antibody

• Forkhead box protein C2 antibody

• Forkhead related protein FKHL14 antibody

• Forkhead-related protein FKHL14 antibody

• FOX C2 antibody

• Foxc2 antibody

• FOXC2_HUMAN antibody

• LD antibody

• Mesenchyme fork head protein 1 antibody

• Mesenchyme Forkhead 1 antibody

• MFH 1 antibody

• MFH 1 protein antibody

• MFH-1 protein antibody

• MFH1 antibody

• Transcription factor FKH 14 antibody

• Transcription factor FKH-14 antibody

• 

  Western blot - Anti-FOXC2 antibody (ab5060)

  Lane 1 : Anti-FOXC2 antibody (ab5060) at 2 µg/ml
  Lane 2 : Anti-FOXC2 antibody (ab5060) at 1 µg/ml
  
  Lane 1 : nuclear HEK293 lysate in RIPA buffer
  Lane 2 : human pancreas whole cell lysate (negative control) in RIPA buffer
  
  Lysates/proteins at 35 µg per lane.
  
  Developed using the ECL technique.
  
  Predicted band size: 53 kDa
  Observed band size: 60-70 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Primary incubation: 1 hour at room temperature.

  Negative Control: Human Pancreas lysate.

• 

  Western blot - Anti-FOXC2 antibody (ab5060)Image courtesy of a review by Michal Bell submitted 19 August 2004

  FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector.