Anti-Cleaved PARP1 antibody

• 产品名称

  Anti-Cleaved PARP1抗体
  参阅全部 Cleaved PARP1 一抗

• 描述

  兔多克隆抗体to Cleaved PARP1

• 宿主

  Rabbit

• 特异性

  This antibody specifically recognizes the 85 kDa fragment of cleaved PARP1 and can be used as marker for detecting apoptotic cells. Cleavage site specific antibody, unconjugated. The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP1 and will recognize Asp 214 and Gly 215.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide corresponding to Human Cleaved PARP1.
  (Peptide available as ab10779)

• 阳性对照

• WB: THP1 Nuclear Enriched, HeLa Nuclear Enriched, KARPAS-299 and Daudi cell lysate. HeLa and Jurkat cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.3
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 50% Glycerol, 0.1% BSA
  
  BSA is IgG and protease free

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a peptide spanning the cleavage site to remove antibody that is reactive with full length PARP1. The final product is generated by affinity chromatography using a peptide corresponding to the PARP1 cleavage site.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4830于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 142 Human

• Omim: 173870 Human

• SwissProt: P09874 Human

• Unigene: 177766 Human

• 别名

• ADPRT 1 antibody

• ADPRT antibody

• ADPRT1 antibody

• APOPAIN antibody

• ARTD1 antibody

• NAD(+) ADP-ribosyltransferase 1 antibody

• PARP antibody

• PARP-1 antibody

• PARP1 antibody

• PARP1_HUMAN antibody

• Poly [ADP-ribose] polymerase 1 (PARP-1) antibody

• Poly [ADP-ribose] polymerase 1 antibody

• Poly ADP ribose polymerase 1 antibody

• Poly(ADP ribose) polymerase antibody

• Poly[ADP-ribose] synthase 1 antibody

• PPOL antibody

• SCA1 antibody

• ADP ribosyltransferase diphtheria toxin like 1 antibody

• ADP ribosyltransferase NAD(+) antibody

• ADP-ribosyltransferase diphtheria toxin-like 1 antibody

• 

  Western blot - Anti-Cleaved PARP1 antibody (ab4830)

  All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : Jurkat cells treated with Etoposide (1 µM for 16 hours)
  Lanes 3 & 5 : HeLa cell lysate
  Lane 4 : HeLa cells treated with Etoposide (1 µM for 16 hours)
  Lane 6 : HeLa cells treated with Staurosporine (3 µM for 16 hours)
  
  Lysates/proteins at 40 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/14000 dilution
  
  Predicted band size: 85 kDa
  
  

• 

  Western blot - Anti-Cleaved PARP1 antibody (ab4830)

  All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/2000 dilution
  
  Lane 1 : THP1 Nuclear Enriched
  Lane 2 : HeLa Nuclear Enriched
  Lane 3 : KARPAS-299
  Lane 4 : Daudi cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
  
  Predicted band size: 85 kDa
  
  

• 

  Western blot - Anti-Cleaved PARP1 antibody (ab4830)This image is courtesy of an Abreview submitted by Adam Szadkowski on 26 January 2006.

  All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution
  
  Lane 1 : Non-induced Jurkat cells
  Lane 2 : Induced Jurkat cells
  
  Secondary
  All lanes : Goat Anti-Rabbit HRP
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 85 kDa
  Observed band size: 85 kDa
  
  
  Exposure time: 5 seconds
  

  See Abreview