Anti-GFAP antibody [2A5]

• 产品名称

  Anti-GFAP抗体[2A5]
  参阅全部 GFAP 一抗

• 描述

  小鼠单克隆抗体[2A5] to GFAP

• 宿主

  Mouse

• 特异性

  This clone gives a much stronger signal in pig, and human samples than in rodents. For rodent (mouse, rat, etc) samples use ab68428.

• 经测试应用

  适用于: IHC-FrFl, WB, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Human, Pig
  

• 免疫原

  Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.

• 阳性对照

• IHC-P: Human brain tissue, human cerebellum tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.065% Sodium azide
  Constituent: Tissue culture supernatant
  
  Concentrated Tissue Culture Supernatant

• 纯度

  Tissue culture supernatant

• 纯化说明

  Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  2A5

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab4648于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 2670 Human

• Entrez Gene: 14580 Mouse

• Entrez Gene: 396562 Pig

• Entrez Gene: 24387 Rat

• Omim: 137780 Human

• SwissProt: P14136 Human

• SwissProt: P03995 Mouse

• SwissProt: P47819 Rat

• Unigene: 514227 Human

• Unigene: 1239 Mouse

• Unigene: 91512 Rat

别名

• wu:fb34h11 antibody

• ALXDRD antibody

• cb345 antibody

• etID36982.3 antibody

• FLJ42474 antibody

• FLJ45472 antibody

• GFAP antibody

• GFAP_HUMAN antibody

• gfapl antibody

• Glial fibrillary acidic protein antibody

• Intermediate filament protein antibody

• wu:fk42c12 antibody

• xx:af506734 antibody

• zgc:110485 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)

  Immunohistochemistry analysis of paraffin-embedded human cerebellum tissue sections labelling GFAP with ab4648 at 1/500 dilution (1 mg/mL). Sections were stained with ab4648 using the HRP/DAB staining. Sections were counterstained with hematoxylin/eosin. Antigen retrieval was heat mediated using Antigen retrieval buffer (100X citrate buffer) (pH 6.0) (ab93678) for 15 minutes.
  To the right is a region of cerebellar molecular layer containing the prominent cytoskeletal fibers of Bergmann glia which are strongly positive for GFAP. The middle shows a region of the granular layer and to the left is white matter, both of which contain GFAP positive astrocytes.The immunostaining was performed with the Vector ImmPress rat adsorbed horse anti-mouse IgG detection kit.

• 

  Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)

  Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.

   

• 

  Western blot - Anti-GFAP antibody [2A5] (ab4648)

  Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution
  
  Lane 1 : MW markers
  Lane 2 : Rat brain tissue lysate
  Lane 3 : Rat spinal cord tissue lysate
  Lane 4 : Mouse brain tissue lysate
  Lane 5 : Mouse spinal cord tissue lysate
  Lane 6 : Pig brain tissue lysate
  Lane 7 : Recombinant rat GFAP protein
  Lane 8 : Recombinant human GFAP protein
  
  Predicted band size: 49 kDa
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

  Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)

  Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
  Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

  Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

  Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

  Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).