Anti-EED抗体
参阅全部 EED 一抗
兔多克隆抗体to EED
Rabbit
适用于: WB, ICC/IFmore details
与反应: Human, Recombinant fragment
预测可用于: Mouse, Chicken, Cow, Xenopus laevis
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
WB: Human Recombinant EED Protein, HAP1, HeLa and HepG2 cell lysates ICC/IF: U-2 OS cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.7 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab4469于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/200. Predicted molecular weight: 49 kDa. |
| ICC/IF | 1/100. |
Entrez Gene: 426381 Chicken
Entrez Gene: 8726 Human
Entrez Gene: 13626 Mouse
Entrez Gene: 398420 Xenopus laevis
Omim: 605984 Human
SwissProt: Q5ZKH3 Chicken
SwissProt: O75530 Human
SwissProt: Q921E6 Mouse
SwissProt: Q8UUP2 Xenopus laevis
Unigene: 503510 Human
Unigene: 380914 Mouse
eed antibody
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Embryonic ECTODERM development protein homolog antibody
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hEED antibody
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WAIT-1 antibody
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Western blot - Anti-EED antibody (ab4469)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EED knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab4469 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab4469 was shown to recognize EED in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when EED knockout samples were examined. Wild-type and EED knockout samples were subjected to SDS-PAGE. ab4469 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-EED antibody (ab4469)
Rabbit polyclonal to EED (ab4469) on U-2 OS (Human bone osteosarcoma epithelial cell line) cells. Antibody used at 1/100.
U-2 OS cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).
Secondary antibody: donkey anti-rabbit (Cy3).
Western blot - Anti-EED antibody (ab4469)
Anti-EED antibody (ab4469) at 1/250 dilution + EED Recombinant Protein (Human) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 80 kDawhy is the actual band size different from the predicted?
ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).
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