Anti-SNAP23抗体
参阅全部 SNAP23 一抗
兔多克隆抗体to SNAP23
Rabbit
This antibody reacts specifically with human SNAP23.
适用于: IHC-Fr, IP, WB, ICC/IF, IHC-Pmore details
与反应: Mouse, Rat, Human
Fusion protein corresponding to Human SNAP23. Immunogen is a SNAP23-GST fusion protein
Database link: O00161
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: Whole serum
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab4114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 17272274 | |
| IP | Use at an assay dependent concentration. PubMed: 22393054 | |
| WB | (1) | Use at an assay dependent concentration. |
| ICC/IF | (1) | Use at an assay dependent concentration. |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 8773 Human
Entrez Gene: 20619 Mouse
Omim: 602534 Human
SwissProt: O00161 Human
SwissProt: O09044 Mouse
Unigene: 511149 Human
Unigene: 245715 Mouse
Unigene: 14789 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab4114)
IHC image of ab4114 staining in human placenta tissue formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
