Anti-E2F1 antibody [KH95]

• 产品名称

  Anti-E2F1抗体[KH95]
  参阅全部 E2F1 一抗

• 描述

  小鼠单克隆抗体[KH95] to E2F1

• 宿主

  Mouse

• 特异性

  Protein of 60 kDa.

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human E2F1.

• 阳性对照

• WB: HepG2, HEK-293, HeLa and U-2 OS cell lysates. IHC-P: Human tonsil tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.05% Sodium azide
  Constituents: Ascites, 1% BSA

• 纯度

  IgG fraction

• 克隆

  单克隆

• 克隆编号

  KH95

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab4070于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1869 Human

• Omim: 189971 Human

• SwissProt: Q01094 Human

• Unigene: 654393 Human

• 别名

• E(Sev-CycE)3A antibody

• E(var)3-93E antibody

• E2-promoter binding facto antibody

• E2F 1 antibody

• E2F transcription factor 1 antibody

• E2F-1 antibody

• E2f-PA antibody

• E2f-PB antibody

• E2f-PC antibody

• E2F1 antibody

• E2f1 E2F transcription factor 1 antibody

• E2F1_HUMAN antibody

• Evar(3)164 antibody

• KIAA4009 antibody

• l(3)07172 antibody

• l(3)j3B1 antibody

• l(3)j3C2 antibody

• l(3)rM729 antibody

• mKIAA4009 antibody

• OTTHUMP00000030661 antibody

• PBR3 antibody

• PRB binding protein E2F 1 antibody

• PRB-binding protein E2F-1 antibody

• RBAP 1 antibody

• RBAP-1 antibody

• RBAP1 antibody

• RBBP-3 antibody

• RBBP3 antibody

• RBP 3 antibody

• RBP3 antibody

• Retinoblastoma-associated protein 1 antibody

• Retinoblastoma-binding protein 3 antibody

• Transcription factor E2F1 antibody

• Dmel\CG6376 antibody

• Dmel_CG6376 antibody

• drosE2F1 antibody

• 

  Western blot - Anti-E2F1 antibody [KH95] (ab4070)

  All lanes : Anti-E2F1 antibody [KH95] (ab4070) at 1/500 dilution
  
  Lane 1 : Wild-type HCT 116 cell lysate
  Lane 2 : E2F1 knockout HCT 116 cell lysate
  Lane 3 : Wild-type HEK-293 ab259780 cell lysate
  Lane 4 : E2F1 knockout HEK-293 ab269502 cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 47 kDa
  Observed band size: 47 kDa
  
  
  
  

  Western blot: Anti-E2F1 antibody [KH95] (ab4070) staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab4070 was shown to bind specifically to E2F1. A band was observed at 47 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in E2F1 knockout cell line. To generate this image, wild-type and E2F1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

• 

  Western blot - Anti-E2F1 antibody [KH95] (ab4070)

  All lanes : Anti-E2F1 antibody [KH95] (ab4070) at 1/500 dilution
  
  Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
  Lane 2 : E2F1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
  Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
  Lane 4 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
  Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 47 kDa
  Observed band size: 55 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Lanes 1 - 5: Merged signal (red and green). Green - ab4070 observed at 55 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

  ab4070 was shown to react with E2F1 in wild-type HEK-293 cells in Western blot with loss of signal observed in E2F1 knockout sample. Wild-type HEK-293 and E2F1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab4070 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)

  ab4070 staining human tonsil by IHC-P.