Anti-BAF53A抗体- ChIP Grade
参阅全部 BAF53A 一抗
兔多克隆抗体to BAF53A - ChIP Grade
Rabbit
In Western blot, ab3882 detects BAF53 and Tip60-TAP in total extracts of Tip60-TAP. Following TEV elution of Tip60-TAP ab3882 only detects BAF53. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: ChIP, WBmore details
与反应: Mouse, Rat, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.5 - 0.8 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3882于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP | Use at an assay dependent concentration. | |
| WB | 1/1000. Detects a band of approximately 53 kDa. |
Entrez Gene: 86 Human
Entrez Gene: 56456 Mouse
Omim: 604958 Human
SwissProt: O96019 Human
SwissProt: Q9Z2N8 Mouse
Actin related protein antibody
Actin related protein Baf53a antibody
actin-related protein 4 antibody
ACTL 6 antibody
ACTL 6A antibody
ACTL6 antibody
ACTL6A antibody
Arp4 antibody
ARPN BETA antibody
ArpNbeta antibody
BAF 53 antibody
BAF 53A antibody
BAF complex 53 kDa subunit antibody
BAF53 antibody
BRG1 associated factor 53A antibody
BRG1 associated factor antibody
BRG1/brm associated factor 53A antibody
hArpN beta antibody
INO80 complex subunit K antibody
INO80K antibody
MGC5382 antibody
53 kDa BRG1 associated factor A antibody
Actin like 6A antibody
Actin like protein 6A antibody
Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)This image is courtesy of Jacques Cote, Universite Laval, Quebec, Canada
All lanes : Anti-BAF53A antibody - ChIP Grade (ab3882) at 1/1000 dilution
Lane 1 : TEV elution of Tip60-TAP clone 1
Lane 2 : TEV elution of Tip60-TAP clone 2
Lane 3 : Total extract of Tip60-TAP clone 1
Lane 4 : Total extract of Tip60-TAP clone 2
Performed under reducing conditions.
Predicted band size: 47 kDa
Total extracts from two different clones of MCF7 (Human breast adenocarcinoma cell line) cells expressing a TAP tagged version of Tip60 were partially purified over IgG sepharose resin and eluted with TEV protease. Western blot was performed using a 1/1000 dilution of the BAF53A antibody.
Lane 1. TEV elution of Tip60-TAP clone #1
Lane 2. TEV elution of Tip60-TAP clone #2
Lane 3. Total extract of Tip60-TAP clone #1
Lane 4. Total extract of Tip60-TAP clone #2
NB: Tip60 is detected in the total extract due to the presence of Protein A in the TAP Tag (to which the primary and secondary abs bind). The TEV protease removes the Tag in lanes 1 and 2.
ChIP - Anti-BAF53A antibody - ChIP Grade (ab3882)This image is courtesy of Sylvain Daujat
Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3882 to BAF53A and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 8 µl of ab3882 and 2x106 cells were used in each ChIP experiment.
Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)
All lanes : Anti-BAF53A antibody - ChIP Grade (ab3882) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 & 3 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Lanes 2 & 4-5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 120 kDa, 18 kDa. We are unsure as to the identity of these extra bands.
