Anti-AMPK alpha 1抗体
参阅全部 AMPK alpha 1 一抗
兔多克隆抗体to AMPK alpha 1
Rabbit
适用于: IP, WB, IHC-Pmore details
与反应: Mouse, Rat, Cow, Human
预测可用于: Orangutan
Synthetic peptide within Human AMPK alpha 1 aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. BAA36547.1 (GeneID 5562).
Database link: Q13131
WB: Bovine aortic endothelial cell extracts. Rat aortic smooth muscle cell extracts. HepG2 extracts. Human aortic endothelial cell extract. HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IHC-P: Human muscle and pancreas tissue. IP: AMPK alpha 1 IP in HeLa cell lysate.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µl 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3759于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | (1) | 1/500 - 1/10000. Predicted molecular weight: 63 kDa. |
| IHC-P | Use at an assay dependent concentration. |
Entrez Gene: 5562 Human
Entrez Gene: 105787 Mouse
Omim: 602739 Human
SwissProt: Q13131 Human
SwissProt: Q5EG47 Mouse
Unigene: 43322 Human
Unigene: 207004 Mouse
Unigene: 87789 Rat
5 AMP activated protein kinase alpha 1catalytic subunit antibody
5 AMP activated protein kinase catalytic alpha 1 chain antibody
5' AMP activated protein kinase catalytic subunit alpha 1 antibody
5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
AAPK1 antibody
AAPK1_HUMAN antibody
ACACA kinase antibody
acetyl CoA carboxylase kinase antibody
AI194361 antibody
AI450832 antibody
AL024255 antibody
AMP -activate kinase alpha 1 subunit antibody
AMP-activate kinase alpha 1 subunit antibody
AMP-activated protein kinase, catalytic, alpha -1 antibody
AMPK 1 antibody
AMPK alpha 1 antibody
AMPK alpha 1 chain antibody
AMPK antibody
AMPK subunit alpha 1 antibody
AMPK subunit alpha-1 antibody
AMPK1 antibody
AMPKa1 antibody
AMPKalpha1 antibody
C130083N04Rik antibody
cb116 antibody
EC 2.7.11.1 antibody
HMG CoA reductase kinase antibody
HMGCR kinase antibody
hormone sensitive lipase kinase antibody
Hydroxymethylglutaryl CoA reductase kinase antibody
im:7154392 antibody
kinase AMPK alpha1 antibody
MGC33776 antibody
MGC57364 antibody
OTTHUMP00000161795 antibody
OTTHUMP00000161796 antibody
PRKAA 1 antibody
PRKAA1 antibody
Protein kinase AMP activated alpha 1 catalytic subunit antibody
SNF1-like protein AMPK antibody
SNF1A antibody
Tau protein kinase PRKAA1 antibody
wu:fa94c10 antibody
Western blot - Anti-AMPK alpha 1 antibody (ab3759)
All lanes : Anti-AMPK alpha 1 antibody (ab3759) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 63 kDa
Exposure time: 3 seconds
Lysated prepared using NETN lysis buffer.
Western blot - Anti-AMPK alpha 1 antibody (ab3759)
Samples: Extracts from
1. Bovine aortic endothelial cells
2. Rat aortic smooth muscle cells
3. HepG2 cells
4. Human aortic endothelial cells
Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling AMPK alpha 1 with ab3759 at 1/5000 (0.2µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)
IHC image of AMPK alpha 1 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunoprecipitation - Anti-AMPK alpha 1 antibody (ab3759)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1 and 2: ab3759 used for IP at 3 µg per reaction, two lots.
Lane 3: Control IgG.
For blotting immunoprecipitated AMPK alpha 1, ab3759 was used at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 seconds.
