Anti-CPS1 antibody

• 产品名称

  Anti-CPS1抗体
  参阅全部 CPS1 一抗

• 描述

  兔多克隆抗体to CPS1

• 宿主

  Rabbit

• 特异性

  When the antibody is used at 1/2000 some lower molecular weight bands appear in COS-7 cells, the extent of which is significantly reduced when the antibody is used at 1/10,000.

• 经测试应用

  适用于: IHC-Fr, IHC-P, IP, ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Rat, African green monkey
  

• 免疫原

  Full length native protein (purified) corresponding to Rat CPS1.

• 常规说明

  OMIM reference: 114010 Alternative name: carbamoylphosphate synthetase 1, CAD

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide

• 纯度

  Whole antiserum

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3682于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 227231 Mouse

• Entrez Gene: 497840 Rat

• SwissProt: Q8C196 Mouse

• SwissProt: P07756 Rat

• Unigene: 343942 Mouse

• Unigene: 53968 Rat

• 别名

• Carbamoyl phosphate synthetase 1 antibody

• Carbamoyl phosphate synthetase 1 mitochondrial antibody

• Carbamoyl phosphate synthetase I antibody

• Carbamoyl-phosphate synthase [ammonia] antibody

• Carbamoyl-phosphate synthetase I antibody

• Carbamoylphosphate synthase antibody

• Carbamoylphosphate synthetase 1 antibody

• Carbamoylphosphate synthetase I antibody

• CPS 1 antibody

• Cps1 antibody

• CPSase 1 antibody

• CPSase I antibody

• CPSASE1 antibody

• CPSM_HUMAN antibody

• mitochondrial antibody

• MS738 antibody

• Carbamoyl phosphate synthase [ammonia] antibody

• Carbamoyl phosphate synthase [ammonia] mitochondrial antibody

• Carbamoyl phosphate synthase antibody

• 

  Western blot - Anti-CPS1 antibody (ab3682)

  Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
  Lane 2: cos-7 cells transfected with full length CPS 1. IP with 5 ul of pre-immune serum
  Lane 3: cos-7 cells transfected with full length CPS 1. IP with 2 ul of polyclonal antibody anti CPS 1 (ab3682)
  Lane 4: cos-7 cells transfected with full length CPS 1. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
  Lane 5: 5 ul of 2ml lysate cos-7 cells transfected with empty vector (pCMV5-tr). Before IP
  Lane 6: cos-7 cells transfected with empty vector (pCMV5-tr). IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
  Lane 7: 5 ul of 2ml lysate untransfected cos-7 cells. Before IP
  Lane 8: untransfected cos-7 cells. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682) Ab3682 can IP CPS1 transiently expressed in COS-7 cells.
  
  Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
  Lane 2: cos-7 cells transfected with full length CPS 1. IP

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)

  CPS-1 localisation is consistent with the reticulated pattern of liver mitonchondria.

  Rabbit polyclonal to CPS-1 (ab3682), at 1/5000 on COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV5-CPS 1 plasmid (Corvi, M.M and Berthiaume, L.G. unpublished data).

  Secondary: donkey anti-rabbit (Texas Red).

• 

  Western blot - Anti-CPS1 antibody (ab3682)

  Western blot using Rabbit polyclonal to CPS 1 (ab3682). All lanes loaded with 100 ng of purified CPS 1
  Lane 1: ab3682 1/500
  Lane 2: ab3682 1/1000
  Lane 3: ab3682 1/2000 Western blot using Rabbit polyclonal to CPS 1 (ab3682).
  
  All lanes loaded with 100 ng of purified CPS 1
  
  Lane 1: ab3682 1/500
  Lane 2: ab3682 1/1000
  Lane 3: ab3682 1/2000