Anti-Furin抗体
参阅全部 Furin 一抗
兔多克隆抗体to Furin
Rabbit
Detects Furin from canine and mouse cells as well as transfected human Furin. This antibody does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.
适用于: ICC, WBmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human Furin aa 780-793.
Sequence:
RGERTAFIKDQSAL
(Peptide available as ab4989)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µl 浓度为 2 mg/ml
Immunogen affinity purified
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
多克隆
IgG
Abpromise™承诺保证使用ab3467于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use a concentration of 2 µg/ml. | |
| WB | (3) | Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa). Representing Furin in BSC-40 cells transfected with the human Furin gene. |
Entrez Gene: 5045 Human
Entrez Gene: 18550 Mouse
Omim: 136950 Human
SwissProt: P09958 Human
SwissProt: P23188 Mouse
Unigene: 513153 Human
Unigene: 5241 Mouse
FUR antibody
FURIN antibody
Furin membrane associated receptor protein antibody
FURIN_HUMAN antibody
PACE antibody
Paired basic amino acid residue cleaving enzyme antibody
Paired basic amino acid residue-cleaving enzyme antibody
PCSK3 antibody
Proprotein convertase subtilisin/kexin type 3 antibody
SPC1 antibody
Dibasic processing enzyme antibody
Dibasic-processing enzyme antibody
FES upstream region antibody
Western blot - Anti-Furin antibody (ab3467)This image is courtesy of an Abreview submitted by Lawrence Welch
All lanes : Anti-Furin antibody (ab3467) at 1/1000 dilution
Lane 1 : HAP1
Lanes 2-3 : independent furin CRISPR KO clones (Horizon)
Lysates/proteins at 250000 cells per lane.
Secondary
All lanes : rabbit IgG HRP Linked Whole Ab at 1/10000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 87 kDa
Observed band size: 100 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
4-20% tris-glycine, denaturing conditions
Immunocytochemistry - Anti-Furin antibody (ab3467)
Immunocytochemical analysis of methanol-fixed 2% BSA blocked HeLa cells strining furin with ab3467 at 2 µg/ml and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei are stained with SlowFade® Gold Antifade Mountant with DAPI (blue), and the cytoskeleton with alpha-Tubulin Monoclonal Antibody and Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate at 1/400 dilution (red). Negative control uses no primary antibody
Western blot - Anti-Furin antibody (ab3467)
All lanes : Anti-Furin antibody (ab3467) at 1/500 dilution
Lane 1 : HeLa lysate at 30 µg
Lane 2 : HEK-293 extract at 30 µg
Lane 3 : K-562 extract at 30 µg
Lane 4 : SH-SY5Y extract at 30 µg
Lane 5 : conditioned media from HeLa cell line at 10 µg
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa
Immunocytochemistry - Anti-Furin antibody (ab3467)
Immunolocalization of endogenous furin in mouse 3T3 cells with ab3467.
Western blot - Anti-Furin antibody (ab3467)
All lanes : Anti-Furin antibody (ab3467) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 63 kDawhy is the actual band size different from the predicted?
Exposure time: 90 seconds
The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide.
Immunocytochemistry - Anti-Furin antibody (ab3467)Image courtesy of an anonymous Abreview.
ab3467 staining Furin in human A549 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA/ 5%FBS/PBS for 1 hour at 25°C and then incubated with ab3467 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
Cells were counterstained with DAPI (436nM, 5 mins) and Phalloidin-633 (1unit/coverslip) and mounted with prolong gold. No staining seen in secondary control and furin staining was punctate and throughout the cytoplasm.
