Anti-CPEB1抗体
参阅全部 CPEB1 一抗
兔多克隆抗体to CPEB1
Rabbit
适用于: IHC-P, ICC/IF, WBmore details
与反应: Mouse, Rat, Human
预测可用于: Xenopus laevis
Synthetic peptide corresponding to Human CPEB1 aa 545-562.
Sequence:
HSMEGLRHHSPLMRNQKN
(Peptide available as ab4988)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3465于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/50 - 1/500. | |
| ICC/IF | (1) | 1/50 - 1/500. |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
Abpromise™承诺保证使用ab3465于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/50 - 1/500. | |
| ICC/IF | (1) | 1/50 - 1/500. |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
Entrez Gene: 64506 Human
Entrez Gene: 12877 Mouse
Entrez Gene: 399289 Xenopus laevis
Entrez Gene: 734470 Xenopus laevis
Omim: 607342 Human
SwissProt: Q9BZB8 Human
SwissProt: P70166 Mouse
Unigene: 547988 Human
Unigene: 273122 Mouse
Unigene: 224485 Rat
CEBP antibody
CPE binding protein 1 antibody
CPE BP1 antibody
CPE-binding protein 1 antibody
CPE-BP1 antibody
CPEB 1 antibody
CPEB antibody
CPEB-1 antibody
CPEB1 antibody
CPEB1 protein antibody
CPEB1_HUMAN antibody
Cytoplasmic polyadenylation binding protein 1 antibody
Cytoplasmic polyadenylation element binding protein antibody
Cytoplasmic polyadenylation element binding protein 1 antibody
Cytoplasmic polyadenylation element-binding protein 1 antibody
FLJ13203 antibody
h CEBP antibody
h-CEBP antibody
hCPEB 1 antibody
hCPEB-1 antibody
MGC34136 antibody
MGC60106 antibody
mKIAA0940 antibody
MKIAA0940 protein antibody
Western blot - Anti-CPEB1 antibody (ab3465)
All lanes : Anti-CPEB1 antibody (ab3465) at 1/500 dilution
Lane 1 : HeLa lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Mouse brain tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 62 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
Western blot - Anti-CPEB1 antibody (ab3465)
Western blot detection of 1.0 ng of recombinant mouse CPEB1 using ab3465.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPEB1 antibody (ab3465)
Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded human hearat tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPEB1 antibody (ab3465)
Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPEB1 antibody (ab3465)
Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunocytochemistry/ Immunofluorescence - Anti-CPEB1 antibody (ab3465)
Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor® 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-CPEB1 antibody (ab3465)
Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-CPEB1 antibody (ab3465)
Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained
