Anti-Adenosine A1 Receptor抗体
参阅全部 Adenosine A1 Receptor 一抗
兔多克隆抗体to Adenosine A1 Receptor
Rabbit
Detects Adenosine Receptor A1. This antibody does not detect other AR subtypes.
适用于: WB, ICC, IHC-Pmore details
与反应: Rat, Human
预测可用于: Cow
Synthetic peptide corresponding to Rat Adenosine A1 Receptor aa 309-326.
Sequence:
CQPKPPIDEDLPEEKAED
(Peptide available as ab5893)
WB: PC-12, SH-SY5Y, U-87MG, A549, K-562 whole cell lysates. ICC: HeLa cells IHC: rat brain tissue.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
浓度
100 µl 浓度为 1 mg/ml
Immunogen affinity purified
Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.
多克隆
IgG
Abpromise™承诺保证使用ab3460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500. Predicted molecular weight: 37 kDa.Can be blocked with Adenosine A1 Receptor peptide (ab5893). | |
| ICC | Use a concentration of 2 µg/ml. | |
| IHC-P | 1/50 - 1/200. |
Entrez Gene: 134 Human
Omim: 102775 Human
SwissProt: P30542 Human
Unigene: 77867 Human
Unigene: 32078 Rat
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Ri antibody
Immunocytochemistry - Anti-Adenosine A1 Receptor antibody (ab3460)
Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized HeLa cells staining adenosine A1 receptor with ab3460 at 2 µg/ml and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei are stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin is stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control contains no primary anibody.
Western blot - Anti-Adenosine A1 Receptor antibody (ab3460)
All lanes : Anti-Adenosine A1 Receptor antibody (ab3460) at 1/250 dilution
Lane 1 : PC-12 whole cell extract
Lane 2 : SH-SY5Y whole cell extract
Lane 3 : U-87 MG whole cell extract
Lane 4 : A549 whole cell extract
Lane 5 : K-562 whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab3460)
ab3460 labelling Adenosine A1 receptor in the cytoplasm of Rat brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
