Anti-Dynamin 2抗体
参阅全部 Dynamin 2 一抗
兔多克隆抗体to Dynamin 2
Rabbit
适用于: WB, ICC/IF, IHC-Pmore details
与反应: Mouse, Rat, Human, Non human primates
预测可用于: Cow
Synthetic peptide corresponding to Human Dynamin 2 aa 760-779.
Sequence:
SPTPQRRPVSSIHPPGRPPA
(Peptide available as ab4985)
WB: SH-SY5Y, PC-12, Neuro 2a, SK-N-AS, U-87 MG, rat brain tissue; ICC/IF: SH-SY5Y, HeLa; IHC: human lung tissue.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µl 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3457于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| ICC/IF | (4) | Use at an assay dependent concentration. |
| IHC-P | Use a concentration of 1 µg/ml. |
Entrez Gene: 1785 Human
Entrez Gene: 13430 Mouse
Omim: 602378 Human
SwissProt: P50570 Human
SwissProt: P39054 Mouse
Unigene: 211463 Human
Unigene: 433257 Mouse
Unigene: 11231 Rat
CMT2M antibody
CMTDI1 antibody
CMTDIB antibody
DI CMTB antibody
Dnm2 antibody
DYN II antibody
DYN2 antibody
DYN2_HUMAN antibody
Dynamin II antibody
Dynamin-2 antibody
Dynamin2 antibody
DynaminII antibody
DYNII antibody
Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y cells labeling Dynamin 2 with ab3457 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green). The nuclear counterstain is DAPI (blue), and F-actin is stained with Rhodamine Phalloidin iat 1/300 dilution (red). Negative control was cells without primary antibody present.
Western blot - Anti-Dynamin 2 antibody (ab3457)
All lanes : Anti-Dynamin 2 antibody (ab3457)
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : PC-12
Lane 3 : Neuro-2a
Lane 4 : SK-N-AS
Lane 5 : U- 87 MG
Lane 6 : Rat Brain
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugated
Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa cells labeling Dynamin 2 with ab3457 at 2 ug/ml followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green).
The nuclear counterstain is DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)
ab3457 (1µg/ml) staining Dynamin 2 in human lung using an automated system (DAKO Autostainer Plus).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
ICC/IF image of ab3457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
