Anti-Mint3抗体
兔多克隆抗体to Mint3
Rabbit
适用于: WB, ICCmore details
与反应: Mouse
Synthetic peptide corresponding to Rat Mint3 aa 1-19.
Sequence:
MEFLPEPQHPPGPPTMDLE
(Peptide available as ab4981)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.
多克隆
IgG
Abpromise™承诺保证使用ab3450于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa). By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract. | |
| ICC | Use a concentration of 2 µg/ml. |
Entrez Gene: 57267 Mouse
SwissProt: O88888 Mouse
Unigene: 293931 Mouse
Amyloid beta precursor protein binding, family A, member 3 phosphotyrosine binding/ interacting domain (PTB) bearing protein antibody
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X11gamma antibody
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amyloid beta (A4) precursor protein binding, family A, member 3 antibody
Amyloid beta A4 precursor protein-binding family A member 3 antibody
Western blot - Anti-Mint3 antibody (ab3450)
Western blot detection of Mint3 on AtT20 cell extract using ab3450.
Immunocytochemistry - Anti-Mint3 antibody (ab3450)
Neuro-2a cells stained for Mint3 (green) using ab3450 at 2 µg/mL in immunocytochemical analysis. Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control.
