Anti-GNB1抗体
参阅全部 GNB1 一抗
兔多克隆抗体to GNB1
Rabbit
适用于: WBmore details
与反应: Rat, Sheep, Human
预测可用于: Dog, Xenopus laevis, Zebrafish, Amphibian, Chinese hamster
Synthetic peptide corresponding to Human GNB1 aa 8-25.
Sequence:
RQEAEQLKNQIRDARKAC
(Peptide available as ab4972)
WB: sheep retinal extracts, rat brain, Jurkat, MC7, MDA-MB-231, T-47D
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
多克隆
IgG
Abpromise™承诺保证使用ab3433于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. |
Entrez Gene: 2782 Human
Entrez Gene: 322104 Zebrafish
Omim: 139380 Human
SwissProt: P62873 Human
SwissProt: P79959 Xenopus laevis
SwissProt: Q6PH57 Zebrafish
Unigene: 430425 Human
Unigene: 126047 Rat
Unigene: 4840 Xenopus laevis
Unigene: 134498 Zebrafish
Beta subunit signal transducing proteins GS/GI antibody
G protein beta 1 subunit antibody
GBB1 antibody
GBB1_HUMAN antibody
gnb1 antibody
Guanine nucleotide binding protein (G protein) beta polypeptide 1 antibody
Guanine nucleotide binding protein beta 1 subunit antibody
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 antibody
Transducin beta chain 1 antibody
Western blot - Anti-GNB1 antibody (ab3433)
All lanes : Anti-GNB1 antibody (ab3433) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 5 : tissue extract of Rat Brain
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Additional bands at: ~37 kDa. We are unsure as to the identity of these extra bands.
Detection: chemiluminescence.
Western blot - Anti-GNB1 antibody (ab3433)
All lanes : Anti-GNB1 antibody (ab3433) at 2 µg/ml
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with skimmed milk
Lane 2 : Rat Brain with skimmed milk
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Detection: chemiluminescence.
Western blot - Anti-GNB1 antibody (ab3433)
Western blot of Tr beta on sheep retinal extracts using ab3433. Western blot of GNB1 on sheep retinal extracts using ab3433.
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