Anti-SCAMP1抗体
兔多克隆抗体to SCAMP1
Rabbit
适用于: WBmore details
与反应: Mouse, Rat, Human
预测可用于: Pig
Synthetic peptide corresponding to Human SCAMP1 aa 1-17.
Sequence:
MSDFDSNPFADPDLNNP
(Peptide available as ab4969)
WB: U-87MG, Neuro-2a, SH-SY5Y, SK-N-AS, T98G, IMR-32, Rat brain tissue and Wild-type HeLa cell lysate.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3430于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa). By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate. |
Entrez Gene: 9522 Human
Entrez Gene: 107767 Mouse
Omim: 606911 Human
SwissProt: O15126 Human
SwissProt: Q8K021 Mouse
Unigene: 482587 Human
Unigene: 201455 Mouse
Unigene: 20374 Rat
SCAM1_HUMAN antibody
SCAMP 1 antibody
SCAMP 37 antibody
SCAMP antibody
SCAMP1 antibody
SCAMP37 antibody
Secretory carrier associated membrane protein 1 antibody
Secretory carrier membrane protein 1 antibody
Secretory carrier membrane protein antibody
Secretory carrier-associated membrane protein 1 antibody
Western blot - Anti-SCAMP1 antibody (ab3430)
All lanes : Anti-SCAMP1 antibody (ab3430) at 1 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SCAMP1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 38 kDa
Observed band size: 36 kDawhy is the actual band size different from the predicted?
Lanes 1 - 2: Merged signal (red and green). Green - ab3430 observed at 36 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab3430 was shown to react with SCAMP1 in wild-type HeLa cells in western blot. The bands observed in SCAMP1 knockout cell line ab265567 (SCAMP1 knockout cell lysate ab258184) below 36 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and SCAMP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3430 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at 1 µg/ml and a 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-SCAMP1 antibody (ab3430)
All lanes : Anti-SCAMP1 antibody (ab3430) at 2 µg/ml
Lane 1 : U-87MG (Human primary glioblastoma cell line) lysate
Lane 2 : Neuro-2a (Mouse brain) lysate
Lane 3 : SH-SY5Y (Human derived cell line) lysate
Lane 4 : SK-N-AS (Human neuroblastoma cell line) lysate
Lane 5 : T98G (Glioblastoma cell line) lysate
Lane 6 : IMR-32 (Human neuroblastoma cell line) lysate
Lane 7 : Rat brain tissue
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 38 kDa
Observed band size: 36 kDawhy is the actual band size different from the predicted?
Blocking buffer: 5% Milk.
Western blot - Anti-SCAMP1 antibody (ab3430)
Western blot detection of SCAMP1 on rat brain extract using ab3430.
