Anti-CASK抗体
参阅全部 CASK 一抗
兔多克隆抗体to CASK
Rabbit
By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with this antibody results in staining of the choroid plexus.
适用于: ICC, WBmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human CASK aa 578-592.
Sequence:
CERDSPSTSRQSPAN
(Peptide available as ab4961)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3383于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC | Use a concentration of 2 µg/ml. | |
WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa. |
Entrez Gene: 8573 Human
Entrez Gene: 12361 Mouse
Omim: 300172 Human
SwissProt: O14936 Human
SwissProt: O70589 Mouse
Unigene: 495984 Human
Unigene: 327591 Mouse
Unigene: 474948 Mouse
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Immunocytochemistry - Anti-CASK antibody (ab3383)
Immunofluorescence analysis of CASK in U-87 MG cells using ab3383. The cells were fixed with 4% paraformaldehyde permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab3383 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. (Panel d) represents the merged image showing cytoplasmic localization. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.
Western blot - Anti-CASK antibody (ab3383)
Western blot detection of CASK on AtT20 cell lysate using ab3383.
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