Anti-BCRP/ABCG2 antibody [BXP-21]

• 产品名称

  Anti-BCRP/ABCG2抗体[BXP-21]
  参阅全部 BCRP/ABCG2 一抗

• 描述

  小鼠单克隆抗体[BXP-21] to BCRP/ABCG2

• 宿主

  Mouse

• 特异性

  BXP-21 reacts with an internal epitope of BCRP/ABCG2, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. This antibody does not react with the human MDR1, MRP1 and MRP2 gene products.

• 经测试应用

  适用于: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, ICCmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment within BCRP/ABCG2 aa 250-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

• 阳性对照

• IHC-P: Human breast cancer tissue. ICC/IF: MCF7 cells. Caco Cell lysate (see second reference cited).

• 常规说明

  For the WB, please note the subcellular localization and distribution of the BCRP/ABCG2 in Normal Human Tissues: PMID:11309308
  
  

  We have received mixed feedback from customers, the antibody showed 45-50 kDa band size in A549 and Caco2 cell lines against predicted size of 72kDa. In response to data we have received we no longer guarantee this antibody for western blot. ABCG2 is a multi-pass membrane protein so for troubleshooting we would recommend heating the samples at 60-70C for 15-20 minutes instead of boiling and as this protein exists as homodimer so complete reduction of samples is highly recommended. In case of any other question please email scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 0.1% BSA, 99.88% PBS

• 纯度

  Tissue culture supernatant

• Primary antibody说明

  Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.

• 克隆

  单克隆

• 克隆编号

  BXP-21

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab3380于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 9429 Human

• Omim: 603756 Human

• SwissProt: Q9UNQ0 Human

• Unigene: 480218 Human

• 别名

• ABCG2 antibody

• ABCG2_HUMAN antibody

• ABCP antibody

• ATP binding cassette sub family G (WHITE) member 2 antibody

• ATP binding cassette transporter G2 antibody

• ATP-binding cassette sub-family G member 2 antibody

• BCRP antibody

• BCRP1 antibody

• BMDP antibody

• Breast cancer resistance protein antibody

• CD338 antibody

• CDw338 antibody

• CDw338 antigen antibody

• EST157481 antibody

• GOUT1 antibody

• MGC102821 antibody

• Mitoxantrone resistance associated protein antibody

• Mitoxantrone resistance-associated protein antibody

• MRX antibody

• Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody

• MXR antibody

• MXR1 antibody

• Placenta specific ATP binding cassette transporter antibody

• Placenta specific MDR protein antibody

• Placenta-specific ATP-binding cassette transporter antibody

• UAQTL1 antibody

• ABC transporter antibody

• ABC15 antibody

• ABCG 2 antibody