Anti-MRP2 antibody [M2 III-6]

• 产品名称

  Anti-MRP2抗体[M2 III-6]
  参阅全部 MRP2 一抗

• 描述

  小鼠单克隆抗体[M2 III-6] to MRP2

• 宿主

  Mouse

• 特异性

  This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Dog

• 免疫原

  Recombinant fragment within Human MRP2 aa 1300-1550 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

• 表位

  This antibody reacts with an internal epitope of MRP 2.

• 常规说明

  We received mixed feedback about rat species so we are removing this from tested species list for now. The immunogen is 84% homologous to human protein so theoretically it should cross react however considering the latest customer feedback we no longer guarantee this species.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituent: 0.1% BSA

• 浓度

• 批次浓度范围 250 µl 浓度为 0.1 - 0.25 mg/ml

• 克隆

  单克隆

• 克隆编号

  M2 III-6

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab3373于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1244 Human

• Omim: 601107 Human

• SwissProt: Q92887 Human

• Unigene: 368243 Human

• 别名

• ATP binding cassette subfamily C member 2 antibody

• ATP-binding cassette sub-family C member 2 antibody

• Canalicular multidrug resistance protein antibody

• Canalicular multispecific organic anion transporter 1 antibody

• CMOAT antibody

• CMOAT1 antibody

• cMRP antibody

• DJS antibody

• KIAA1010 antibody

• MRP 2 antibody

• MRP2_HUMAN antibody

• Multidrug resistance associated protein 2 antibody

• Multidrug resistance-associated protein 2 antibody

• ABC30 antibody

• abcC2 antibody

• ATP binding cassette sub family C (CFTR/MRP) member 2 antibody

• 

  Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)

  All lanes : Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/20 dilution
  
  Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
  Lane 2 : ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
  Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 174 kDa
  
  
  
  

  Lanes 1 - 3: Merged signal (red and green). Green - ab3373 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.

  ab3373 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab3373 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)This image is courtesy of an anonymous Abreview

  Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/500 dilution + WIF-B cells, the hepatoma-derived hybrid cell line at 30 µg with 5% milk in PBST
  
  Secondary
  HRP conjugated Donkey anti-Mouse at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 174 kDa
  Observed band size: 130-250 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 20 minutes
  

  See Abreview