Anti-BMAL1 antibody

• 产品名称

  Anti-BMAL1抗体
  参阅全部 BMAL1 一抗

• 描述

  兔多克隆抗体to BMAL1

• 宿主

  Rabbit

• 特异性

  Detects BMAL 1 / aryl hydrocarbon nuclear translocator 3 (ARNT 3) from hamster and mouse tissues as well as recombinant human BMAL 1.

• 经测试应用

  适用于: WB, ICCmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Horse, Chicken, Syrian hamster

• 免疫原

  Synthetic peptide corresponding to Mouse BMAL1 aa 582-594.
  Sequence:

  DMIDNDQGSSSPS

  
  (Peptide available as ab4959)

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: human U251 and U87-MG, mouse NIH-3T3 cell lines; human SH-SY5Y, U-87 MG, U-2 OS, HeLa, HEK293,and JURKAT cells, and rat brain tissue; ICC: SH-SY5Y cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, PBS

• 浓度

• 200 µl 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3350于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 374115 Chicken

• Entrez Gene: 406 Human

• Entrez Gene: 11865 Mouse

• Entrez Gene: 29657 Rat

• Omim: 602550 Human

• SwissProt: O00327 Human

• SwissProt: Q9WTL8 Mouse

• SwissProt: Q9EPW1 Rat

• Unigene: 65734 Human

• Unigene: 33970 Mouse

• Unigene: 440371 Mouse

• Unigene: 14532 Rat

别名

• ARNT like protein 1 brain and muscle antibody

• Arntl antibody

• Aryl hydrocarbon receptor nuclear translocator like antibody

• Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody

• Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody

• Basic helix loop helix PAS orphan MOP3 antibody

• Basic helix loop helix PAS protein MOP3 antibody

• Basic-helix-loop-helix-PAS protein MOP3 antibody

• bHLH PAS protein JAP3 antibody

• bHLH-PAS protein JAP3 antibody

• bHLHe5 antibody

• BMAL 1 antibody

• BMAL1_HUMAN antibody

• BMAL1c antibody

• Brain and muscle ARNT like 1 antibody

• Brain and muscle ARNT-like 1 antibody

• CG8727 PA antibody

• Class E basic helix-loop-helix protein 5 antibody

• cycle antibody

• JAP 3 antibody

• JAP3 antibody

• Member of PAS protein 3 antibody

• Member of PAS superfamily 3 antibody

• MGC47515 antibody

• MOP 3 antibody

• MOP3 antibody

• PAS domain-containing protein 3 antibody

• PASD 3 antibody

• PASD3 antibody

• TIC antibody

• 

  Immunocytochemistry - Anti-BMAL1 antibody (ab3350)

  Immunocytochemistry analysis of  4% paraformaldehyde-fixed SH-SY5Y cells permeabilised with 0.1% Triton™ X-100 (10 mins) and blocked with 1% BSA (1  hour).

  a) BMAL1 is stained with ab3350 at 2µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate in 1/2000 dilution (green). b) Nuclear staining with DAPI and SlowFade® Gold Antifade Mountant (blue). c) F-actin stainined with a 1/3000 dilution Alexa Fluor® 555 Rhodamine Phalloidin (red). d) merged image. e) control with no primary antibody.

• 

  Western blot - Anti-BMAL1 antibody (ab3350)

  All lanes : Anti-BMAL1 antibody (ab3350) at 1/500 dilution
  
  Lane 1 : U251 cell lysate
  Lane 2 : U87-MG cell lysate
  Lane 3 : NIH-3T3 cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Predicted band size: 69.4 kDa
  Observed band size: 69 kDawhy is the actual band size different from the predicted?
  
  

• 

  Western blot - Anti-BMAL1 antibody (ab3350)

  Anti-BMAL1 antibody (ab3350) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 69.4 kDa
  Observed band size: 75 kDawhy is the actual band size different from the predicted?
  Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 3 minutes
  
  
  The 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BMAL1.