Anti-Proteasome 20S LMP2抗体
参阅全部 Proteasome 20S LMP2 一抗
兔多克隆抗体to Proteasome 20S LMP2
Rabbit
适用于: WBmore details
与反应: Mouse, Human
预测可用于: Rat, Cow
Synthetic peptide corresponding to Human Proteasome 20S LMP2 aa 206-219.
Sequence:
RVILGNELPKFYDE
WB: HeLa cells, mouse embryo fibroblast cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3328于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (5) | Use a concentration of 1 µg/ml. (worked at 1/2000 (PMID 17114438)) |
Entrez Gene: 5698 Human
Entrez Gene: 16912 Mouse
Omim: 177045 Human
SwissProt: P28065 Human
SwissProt: P28076 Mouse
Unigene: 654585 Human
Unigene: 390983 Mouse
Unigene: 13686 Rat
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Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
All lanes : Anti-Proteasome 20S LMP2 antibody (ab3328) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/mL IFN gamma for 72 hours, whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) treated with 50 ng/mL PMA for 48 hours and 200 ng/mL LPS for 24 hours, whole cell lysate
Lane 5 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 6 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 7 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 0.4 µg/ml
Developed using the ECL technique.
Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.