Anti-MiTF antibody [D5]

• 产品名称

  Anti-MiTF抗体[D5]
  参阅全部 MiTF 一抗

• 描述

  小鼠单克隆抗体[D5] to MiTF

• 宿主

  Mouse

• 经测试应用

  适用于: WB, Flow Cyt (Intra), ICCmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Dog不与反应: Rat

• 免疫原

  Fusion protein. This information is proprietary to Abcam and/or its suppliers.

• 表位

  N-terminal

• 阳性对照

• Flow Cyt (Intra): Malme-3 cells. ICC: Malme-3M cells. WB: HeLa whole cell and nuclear lysate.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  D5

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab3201于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 4286 Human

• Entrez Gene: 17342 Mouse

• Omim: 156845 Human

• SwissProt: O75030 Human

• SwissProt: Q08874 Mouse

• Unigene: 166017 Human

• Unigene: 618266 Human

• Unigene: 333284 Mouse

• Unigene: 454504 Mouse

别名

• BHLHE32 antibody

• bHLHe32 antibody

• Class E basic helix-loop-helix protein 32 antibody

• CMM8 antibody

• Homolog of mouse microphthalmia antibody

• Mi antibody

• Microphthalmia associated transcription factor antibody

• Microphthalmia, mouse, homolog of antibody

• Microphthalmia-associated transcription factor antibody

• MITF antibody

• MITF_HUMAN antibody

• mitfa antibody

• nacre antibody

• WS2 antibody

• WS2A antibody

• z3A.1 antibody

• 

  Immunocytochemistry - Anti-MiTF antibody [D5] (ab3201)

  ab3201 staining MiTF in Malme-3M cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab3201 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Western blot - Anti-MiTF antibody [D5] (ab3201)

  All lanes : Anti-MiTF antibody [D5] (ab3201) at 5 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
  Lane 2 : HeLa nuclear lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 59 kDa
  Observed band size: 40 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 10 minutes
  

• 

  Immunocytochemistry - Anti-MiTF antibody [D5] (ab3201)This image is courtesy of an anonymous Abreview

  ab3201 staining MiTF in the SK-MEL-28 (Human cutaneous melanoma cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde and permeabilized with Triton X-100 0.5% in PBS. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/1000) was used as the secondary antibody. SK-MEL-28 cells was grown on chamber slide. MiTF was found to strictly localised in the nucleus. Cells were counter-stained with DAPI.

  See Abreview

• 

  Flow Cytometry (Intracellular) - Anti-MiTF antibody [D5] (ab3201)

  Overlay histogram showing Malme-3 (Human skin fibroblast cell line) cells stained with ab3201 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3201, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.