Anti-HSF1 antibody

• 产品名称

  Anti-HSF1抗体
  参阅全部 HSF1 一抗

• 描述

  兔多克隆抗体to HSF1

• 宿主

  Rabbit

• 经测试应用

  适用于: IP, WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human, Caenorhabditis elegans
  

• 免疫原

  Recombinant full length protein corresponding to Human HSF1. Recombinant human HSF1 expressed in E. coli.

• 阳性对照

• WB: HEK-293T, HeLa, K562, A431, and HepG2 whole cell lysate. C. elegans whole worm tissue. ICC/IF: HeLa and NIH3T3 cells. IP: HeLa whole cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 产品名称

  Anti-HSF1抗体
  参阅全部 HSF1 一抗

• 描述

  兔多克隆抗体to HSF1

• 宿主

  Rabbit

• 经测试应用

  适用于: IP, WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human, Caenorhabditis elegans
  

• 免疫原

  Recombinant full length protein corresponding to Human HSF1. Recombinant human HSF1 expressed in E. coli.

• 阳性对照

• WB: HEK-293T, HeLa, K562, A431, and HepG2 whole cell lysate. C. elegans whole worm tissue. ICC/IF: HeLa and NIH3T3 cells. IP: HeLa whole cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: 99% PBS

• 纯度

  Whole antiserum

• Primary antibody说明

  All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2923于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3297 Human

• Entrez Gene: 15499 Mouse

• Omim: 140580 Human

• SwissProt: Q00613 Human

• SwissProt: P38532 Mouse

• Unigene: 530227 Human

• Unigene: 347444 Mouse

• 别名

• HSF 1 antibody

• hsf1 antibody

• HSF1_HUMAN antibody

• HSTF 1 antibody

• HSTF1 antibody

• Heat shock factor 1 antibody

• Heat shock factor protein 1 antibody

• Heat shock transcription factor 1 antibody

• 

  Western blot - Anti-HSF1 antibody (ab2923)

  All lanes : Anti-HSF1 antibody (ab2923) at 1/1000 dilution
  
  Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
  Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
  Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
  Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
  Lane 6 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
  Lane 7 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
  Lane 8 : NRK (Rat kidney normal tissue) whole cell lysate
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  All lanes : HRP-conjugated goat anti-rabbit IgG secondary antibody at 1/20000 dilution
  
  Predicted band size: 57 kDa
  
  
  
  

  Western blot analysis of Heat Shock Factor 1 (HSF1) was performed by loading samples onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was incubated with ab2923 overnight at 4°C on a rocking platform, washed in TBS-0.1% Tween 20, and probed with a secondary antibody for at least one hour. Chemiluminescent detection was performed.

• 
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HSF1 antibody (ab2923)

  Immunocytochemistry/Immunofluorescence analysis of HSF1 (green) in HeLa (Human epithelial cell line from cervix adenocarcinoma) and NIH/3T3 (Mouse embryo fibroblast cell line) cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with ab2923 (1:50) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat-anti rabbit IgG secondary antibody (1:400) for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

• 

  Immunoprecipitation - Anti-HSF1 antibody (ab2923)

  Immunoprecipitation of HSF1 was performed on HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Antigen:antibody complexes were formed by incubating 500µg whole cell lysate with 2µg of ab2923 overnight on a rocking platform at 4°C. Immune complexes were captured on 50µl Protein A/G Plus Agarose, washed extensively, and eluted with buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was incubated with ab2923 (1:1000) overnight rotating at 4°C, washed in TBST, and probed with detection reagent (1:1000) for at least one hour. Chemiluminescent detection was performed.

• 

  Western blot - Anti-HSF1 antibody (ab2923)

  All lanes : Anti-HSF1 antibody (ab2923) at 1/5000 dilution
  
  Lane 1 : HSF1 RNAi treated C. elegans whole worm tissue
  Lane 2 : Untreated C. elegans whole worm tissue
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : anti-rabbit IgG HRP-linked at 1/5000 dilution
  
  Predicted band size: 57 kDa
  
  
  
  

  Western blot analysis of HSF1 was performed by loading each sample onto an SDS-PAGE gel. Proteins were transferred to PVDF membrane and followed by blocking in TBST+5% non-fat milk. HSF1 was detected using the primary anitbody (ab2923) in TBST+2% non-fat milk overnight at 4°C on a rocking platform, followed by secondary antibody for 30 min. Detection was performed using and 1-Step™ TMB-Blotting Substrate Solution.