Anti-RASA1 antibody [B4F8]

• 产品名称

  Anti-RASA1抗体[B4F8]
  参阅全部 RASA1 一抗

• 描述

  小鼠单克隆抗体[B4F8] to RASA1

• 宿主

  Mouse

• 特异性

  This antibody is specific for RASA1 (an alternate name of GAP).

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Non human primates

• 免疫原

  Recombinant full length protein corresponding to Human RASA1.

• 表位

  Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3.

• 阳性对照

• ICC: C2C12, HeLa, A431; WB: HeLa, A431, mouse brain lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• Primary antibody说明

  GTPase Activating Protein (GAP) is capable of stimulating the hydrolysis of GTP by ras p21 proteins, though GAP has little effect on the oncogenic forms of ras. It is also known that several tyrosine kinases such as platelet derived growth factor receptor and epidermal growth factor receptor are involved in the tyrosine phosphorylation of GAP. It has therefore been suggested that GAP may provide a connection or link between growth factor receptors and the ras p21 family.

• 克隆

  单克隆

• 克隆编号

  B4F8

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab2922于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5921 Human

• Entrez Gene: 218397 Mouse

• Omim: 139150 Human

• SwissProt: P20936 Human

• Unigene: 664080 Human

• 别名

• GAP antibody

• GTPase activating protein antibody

• GTPase-activating protein antibody

• OTTHUMP00000222390 antibody

• OTTHUMP00000222391 antibody

• OTTHUMP00000222392 antibody

• OTTHUMP00000222393 antibody

• p120GAP antibody

• p120RASGAP antibody

• PKWS antibody

• Ras GTPase-activating protein 1 antibody

• RAS p21 protein activator (GTPase activating protein) 1 antibody

• Ras p21 protein activator antibody

• RASA antibody

• RASA1 antibody

• RASA1_HUMAN antibody

• RasGAP antibody

• Triphosphatase activating protein antibody

• CM AVM antibody

• CMAVM antibody

• DKFZp434N071 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

  ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

  ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

  ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• 

  Western blot - Anti-RASA1 antibody [B4F8] (ab2922)

  All lanes : Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : A431 cell lysate
  Lane 3 : Mouse brain cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Observed band size: 110 kDawhy is the actual band size different from the predicted?

•