Anti-Calreticulin抗体- ER Marker
参阅全部 Calreticulin 一抗
兔多克隆抗体to Calreticulin - ER Marker
Rabbit
适用于: ICC/IF, WBmore details
与反应: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Calreticulin.
WB: HL-60, LNCaP, HeLa and MCF-7 cell lysates; Mouse and rat liver tissue lysates; Mouse skeletal muscle whole cell lysate. ICC/IF: A431, HeLa, U2OS, HepG2 and HMVEC cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab2907于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (8) | Use at an assay dependent concentration. |
| WB | (4) | 1/1000. |
Entrez Gene: 811 Human
Entrez Gene: 12317 Mouse
Omim: 109091 Human
SwissProt: P27797 Human
SwissProt: P14211 Mouse
Unigene: 515162 Human
Unigene: 1971 Mouse
Unigene: 467043 Mouse
Unigene: 974 Rat
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Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
All lanes : Anti-Calreticulin antibody - ER Marker (ab2907) at 1/1000 dilution
Lane 1 : HL-60 cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF-7 cell lysate
Lane 5 : Mouse liver tissue lysate
Lane 6 : Rat liver tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Observed band size: 55 kDawhy is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Image from Yount JS et al, J Biol Chem. 2012 Jun 1;287(23):19631-41. Epub 2012 Apr 17, Fig 3. DOI 10.1074/jbc.M112.362095 June 1, 2012 The Journal of Biological Chemistry, 287, 19631-19641.
ab2907 used at a 1/1000 dilution staining Calreticulin in HeLa cells by Immunocytochemistry/ Immunofluorescence.HeLa cells were transfected overnight with empty vector or plasmids encoding the indicated IFITM3 constructs. Immunofluorescence with a-HA antibodies allowed IFITM3 visualization, and a-calreticulin staining allowed visualization of the ER. TOPRO-3 was used to visualize nuclei. Scale bars indicate 10 µm. UbΔ indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine.
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (1:120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.
Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)This image is courtesy of an anonymous Abreview
All lanes : Anti-Calreticulin antibody - ER Marker (ab2907) at 1/1000 dilution
All lanes : Whole cell lysate prepared from mouse skeletal muscle
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated mouse polyclonal to rabbit Ig at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 seconds
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
Immunocytochemistry/Immunofluorescence analysis of Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (1:300) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
Immunocytochemsitry/Immunofluorescence analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X for 30 minutes at room temperature. Cells were incubated with ab2907 (1:50) for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (1:300) in PBS and incubated for 30 minutes. Nuclei (blue) were stained with Hoechst 33342 dye (1µg/mL). Images were taken at 20X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (1:120) and nuclei (red) were stained with DRAQ5 (1ug/ml) for 30 minutes. Images were taken at 20X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (1:120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labelling Calreticulin using ab2907.
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)This image is courtesy of an anonymous Abreview
Immunofluorescence analysis of HepG2 cells, staining Calreticulin with ab2907.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin and blocked with 10% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% saponin) for 1 hour at 20°C. An AlexaFluor®647-conjugated donkey anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.
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