Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3]

• 产品名称

  Anti-alpha 1 Sodium Potassium ATPase抗体[M8-P1-A3]
  参阅全部 alpha 1 Sodium Potassium ATPase 一抗

• 描述

  小鼠单克隆抗体[M8-P1-A3] to alpha 1 Sodium Potassium ATPase

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-P, ICC/IF, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length protein. This information is proprietary to Abcam and/or its suppliers.

• 表位

  This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.

• 阳性对照

• WB: canine kidney extract

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  M8-P1-A3

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2872于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 476 Human

• Omim: 182310 Human

• SwissProt: P05023 Human

• Unigene: 371889 Human

• 别名

• ATP1A1 antibody

• Atpa-1 antibody

• ATPase Na+/K+ transporting alpha 1 polypeptide antibody

• ATPase Na+/K+ transporting subunit alpha 1 antibody

• BC010319 antibody

• EC 3.6.3.9 antibody

• MGC3285 antibody

• MGC38419 antibody

• MGC51750 antibody

• Na K ATPase alpha A catalytic polypeptide antibody

• Na K ATPase catalytic subunit alpha A protein antibody

• Na(+)/K(+) ATPase 1 antibody

• Na(+)/K(+) ATPase alpha-1 subunit antibody

• Na+, K+ ATPase alpha subunit antibody

• Na+/K+ ATPase alpha 1 subunit antibody

• Na+/K+ ATPase 1 antibody

• Na,K ATPase alpha 1 subunit antibody

• Nkaa1b antibody

• Sodium potassium ATPase alpha 1 polypeptide antibody

• Sodium pump 1 antibody

• Sodium pump subunit alpha-1 antibody

• sodium-potassium ATPase catalytic subunit alpha-1 antibody

• Sodium/potassium-transporting ATPase subunit alpha-1 antibody

• A1A1 antibody

• AT1A1 antibody

• AT1A1_HUMAN antibody

• 

  Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

  All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872) at 1/1000 dilution
  
  Lane 1 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
  Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
  Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
  
  Predicted band size: 110 kDa
  
  
  
  

  Samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System.

  Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

  Immunocytochemistry/Immunofluorescence analysis of alpha 1 Sodium Potassium ATPase (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were incubated with ab2872 (1:100) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (1:500) for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)This image is courtesy of an anonymous Abreview

  ab2872 staining pig hepatocyte tissue sections by IHC-P.  The section was fixed with Bouins and subjected to heat mediated antigen retrieval (at pH 9) prior to incubating with the primary antibody, diluted 1/2000, for 1 hour at 20°C.  A Cy3® conjugated goat anti-mouse IgG antibody was used as the secondary.

  See Abreview

• 

  Flow Cytometry - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

  Overlay histogram showing HEK293 cells stained with ab2872 (red line). The cells were fixed with 100% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2872, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde used under the same conditions.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human kidney tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

  All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872) at 1/500 dilution
  
  Lane 1 : Human brain normal tissue lysate - membrane extract (ab29456)
  Lane 2 : Human testis tissue lysate - total protein (ab30257)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 110 kDa
  Observed band size: 100 kDawhy is the actual band size different from the predicted?
  Additional bands at: 25 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 8 minutes
  
  
  The 100 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to alpha 1 Sodium Potassium ATPase.