Anti-Acetylcholinesterase antibody [HR2]

• 产品名称

  Anti-Acetylcholinesterase抗体[HR2]
  参阅全部 Acetylcholinesterase 一抗

• 描述

  小鼠单克隆抗体[HR2] to Acetylcholinesterase

• 宿主

  Mouse

• 特异性

  This antibody does not detect butyrylcholinesterase (BChE).

• 经测试应用

  适用于: ELISA, Flow Cyt, IHC-Fr, IHC-P, ICC/IF, IPmore details
  不适用于: WB

• 种属反应性

  与反应: Mouse, Rabbit, Guinea pig, Cow, Cat, Human, Macaque monkey
  预测可用于: Non human primates不与反应: Rat, Amphibian

• 免疫原

  Full length native protein (purified) corresponding to Human Acetylcholinesterase. Purified Human cerebellar acetylcholinesterase.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 200 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  HR2

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab2803于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for WB.

数据库链接

• Entrez Gene: 540446 Cow

• Entrez Gene: 43 Human

• Entrez Gene: 11423 Mouse

• Omim: 100740 Human

• SwissProt: P23795 Cow

• SwissProt: P22303 Human

• SwissProt: P21836 Mouse

• SwissProt: Q29499 Rabbit

• Unigene: 154495 Human

• Unigene: 255464 Mouse

别名

• ACEE antibody

• ACES_HUMAN antibody

• Acetylcholinesterase antibody

• AChE antibody

• Apoptosis related acetylcholinesterase antibody

• ARACHE antibody

• N ACHE antibody

• N-ACHE antibody

• YT antibody

• YT blood group antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

  Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in HeLa cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

  Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in Neuro-2a cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

  Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in U251 cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Rectum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Flow Cytometry - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

  Overlay histogram showing HeLa cells stained with ab2803 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2803, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.