Anti-Hsp27 antibody [G3.1]

• 产品名称

  Anti-Hsp27抗体[G3.1]
  参阅全部 Hsp27 一抗

• 描述

  小鼠单克隆抗体[G3.1] to Hsp27

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-P, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human Hsp27. (Partially purified human HSP27)

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 1st November 2024.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)

• 浓度

• 100 µg 浓度为 0.2 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  G3.1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2790于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3315 Human

• Omim: 602195 Human

• SwissProt: P04792 Human

• Unigene: 520973 Human

• 别名

• DKFZp586P1322 antibody

• epididymis secretory protein Li 102 antibody

• Estrogen regulated 24 kDa protein antibody

• Estrogen-regulated 24 kDa protein antibody

• Heat shock 25kDa protein 1 antibody

• Heat shock 27 kDa protein antibody

• Heat shock 27kD protein 1 antibody

• Heat shock 27kDa protein 1 antibody

• Heat shock 28kDa protein 1 antibody

• Heat Shock Protein 27 antibody

• Heat shock protein beta 1 antibody

• Heat shock protein beta-1 antibody

• heat shock protein family B (small) member 1 antibody

• HEL-S-102 antibody

• HMN2B antibody

• HS.76067 antibody

• Hsp 25 antibody

• HSP 27 antibody

• Hsp 28 antibody

• Hsp B1 antibody

• Hsp25 antibody

• HSP27 antibody

• Hsp28 antibody

• HspB1 antibody

• HSPB1_HUMAN antibody

• SRP27 antibody

• Stress responsive protein 27 antibody

• Stress-responsive protein 27 antibody

• Heat shock 27kDa protein antibody

• 28 kDa heat shock protein antibody

• CMT2F antibody

• 

  Western blot - Anti-Hsp27 antibody [G3.1] (ab2790)

  Anti-Hsp27 antibody [G3.1] (ab2790) + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  
  Observed band size: 27 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [G3.1] (ab2790)

  Immunohistochemistry of paraffin embedded human breast carcinoma with ab2790 labeling Hsp27 at 0.5μg/ml.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [G3.1] (ab2790)

  Immunohistochemistry of paraffin embedded human prostate carcinoma with ab2790 labeling Hsp27 at 0.5μg/ml.

   

   

• 

  Western blot - Anti-Hsp27 antibody [G3.1] (ab2790)This image is courtesy of an Abreview submitted by Brian Hitt

  Anti-Hsp27 antibody [G3.1] (ab2790) at 1/1000 dilution + Mouse whole brain tissue lysate. at 10 µg
  
  Secondary
  An HRP-conjugated Goat polyclonal. at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Observed band size: 24 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 5 minutes
  
  
  Blocking Step: 5% Milk for 1 hour at 25°C.
  Gel Running Conditions: Reduced, Denaturing Bis-tris 4-12%

  See Abreview

• 

  Flow Cytometry - Anti-Hsp27 antibody [G3.1] (ab2790)

  Overlay histogram showing HeLa cells (ab150035) stained with ab2790 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481)  / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2790, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.