Anti-Calcium Pump PMCA4 ATPase antibody [JA9]

• 产品名称

  Anti-Calcium Pump PMCA4 ATPase抗体[JA9]
  参阅全部 Calcium Pump PMCA4 ATPase 一抗

• 描述

  小鼠单克隆抗体[JA9] to Calcium Pump PMCA4 ATPase

• 宿主

  Mouse

• 特异性

  This antibody is specific to the PMCA4 isoform.

• 经测试应用

  适用于: ICC, Flow Cyt, WB, IHC-Pmore details

• 种属反应性

  与反应: Cow, Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human Calcium Pump PMCA4 ATPase. Purified human erythrocyte PMCA.

• 阳性对照

• WB: SH-SY5Y and HeLa cell lysates.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/m

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  JA9

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2783于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 282146 Cow

• Entrez Gene: 493 Human

• Omim: 108732 Human

• SwissProt: P23634 Human

• Unigene: 343522 Human

• 别名

• ATPase Ca++ transporting plasma membrane 4 antibody

• Cation transporting ATPase antibody

• DKFZp686G08106 antibody

• DKFZp686M088 antibody

• Matrix remodelling associated 1 antibody

• Matrix remodelling associated protein 1 antibody

• Matrix-remodeling-associated protein 1 antibody

• MXRA 1 antibody

• MXRA1 antibody

• OTTHUMP00000034088 antibody

• OTTHUMP00000034089 antibody

• Plasma membrane calcium ATPase 4 antibody

• Plasma membrane calcium ATPase isoform 4 antibody

• Plasma membrane calcium pump isoform 4 antibody

• Plasma membrane calcium transporting ATPase 4 antibody

• Plasma membrane calcium-transporting ATPase 4 antibody

• PMCA 4 antibody

• PMCA4 antibody

• PMCA4b antibody

• PMCA4x antibody

• Sarcolemmal calcium pump antibody

• AT2B4_HUMAN antibody

• ATP2B2 antibody

• Atp2b4 antibody

• 

  Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

  All lanes : Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783) at 1/500 dilution
  
  Lane 1 : Wild-type HeLa cell lysate
  Lane 2 : ATP2B4 knockout HeLa cell lysate
  Lane 3 : SH-SY5Y cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Observed band size: 140 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Lanes 1-3: Merged signal (red and green). Green - ab2783 observed at 140 kDa. Red - loading control, ab181602 observed at 37 kDa.

  ab2783 Anti-Calcium Pump PMCA4 ATPase antibody [JA9] was shown to specifically react with Calcium Pump PMCA4 ATPase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264720 (knockout cell lysate ab258321) was used. Wild-type and Calcium Pump PMCA4 ATPase knockout samples were subjected to SDS-PAGE. ab2783 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

  IHC image of ab2783 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2783, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Flow Cytometry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

  Overlay histogram showing HeLa cells stained with ab2783 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2783, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing PMCA4 ATPase ab2783 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunocytochemistry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

  Immunolocalization of Calcium Pump PMCA4 ATPase in human corneal epithelium.

  (A) Immunolocalization of PMCA4 (green) using ab2783 on cell membranes of all cells in each layer of human corneal epithelium.

  (B) Immunolocalization of Calcium Sensing Receptor (red) using ab19347 on cell membranes of human corneal epithelium.

  (C) Merged image.

• 

  Immunocytochemistry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

  Immunolocalization of Calcium Pump PMCA4 and Calcium Sensing Receptor in bovine corneal epithelium (bCE).

  (A) Immunolocalization of Calcium Pump PMCA4 (green) using ab2783 on cell membranes of all cells in each layer of bCE.

  (B) Immunolocalization of Calcium Sensing Receptor (red) using ab19347 on cell membranes of bCE mostly in wing- and squamous cell layers.

  (C) Merged image.