Anti-KLF15抗体
参阅全部 KLF15 一抗
山羊多克隆抗体to KLF15
Goat
适用于: IP, WB, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat, Pig
Synthetic peptide within Human KLF15 aa 1-100 (N terminal) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)
We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab2647于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. PubMed: 24407292 | |
| WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa). A 1 hour primary incubation is recommended for this product. Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31). Read More | |
| IHC-P | Use at an assay dependent concentration. PubMed: 26378916 |
Entrez Gene: 28999 Human
Entrez Gene: 66277 Mouse
GenBank: NP_054798.1 Human
Omim: 606465 Human
SwissProt: Q9UIH9 Human
SwissProt: Q9EPW2 Mouse
Unigene: 272215 Human
Unigene: 41389 Mouse
Kidney enriched Kruppel like factor antibody
Kidney-enriched krueppel-like factor antibody
KKLF antibody
KKLF protein antibody
KLF 15 antibody
KLF15 antibody
KLF15_HUMAN antibody
Krueppel-like factor 15 antibody
Kruppel like factor 15 antibody
Western blot - Anti-KLF15 antibody (ab2647)
Lane 1 : Anti-KLF15 antibody (ab2647) at 1 µg/ml
Lane 2 : Anti-KLF15 antibody (ab2647) at 0.5 µg/ml
Lane 1 : HepG2 nuclear lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 44 kDa
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