Anti-Cytokeratin 8 antibody [C-43]

• 产品名称

  Anti-Cytokeratin 8抗体[C-43]
  参阅全部 Cytokeratin 8 一抗

• 描述

  小鼠单克隆抗体[C-43] to Cytokeratin 8

• 宿主

  Mouse

• 特异性

  This antibody recognises the 52.5kDa Cytokeratin 8.

• 经测试应用

  适用于: WB, ICC/IF, IHC-P, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  预测可用于: Sheep, Rabbit, Cow, Pig不与反应: Mouse, Rat, Chicken, Hamster, Xenopus laevis

• 免疫原

  Tissue, cells or virus corresponding to Human Cytokeratin 8. Cytoskeleton preparation from HeLa human cervix carcinoma cell line

• 阳性对照

• IHC-P: Human lung FFPE tissue sections. ICC/IF: HepG2 cells WB: HeLa cell lysate Flow Cyt (Intra): MCF7 cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from tissue culture supernatant. Purity >95% by SDS-PAGE.

• 克隆

  单克隆

• 克隆编号

  C-43

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2530于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 281269 Cow

• Entrez Gene: 3856 Human

• Entrez Gene: 100152077 Pig

• Entrez Gene: 100348891 Rabbit

• Entrez Gene: 101110495 Sheep

• Omim: 148060 Human

• SwissProt: P05786 Cow

• SwissProt: P05787 Human

• Unigene: 533782 Human

• Unigene: 708445 Human

别名

• CARD2 antibody

• CK 8 antibody

• CK-8 antibody

• CK8 antibody

• CYK8 antibody

• CYKER antibody

• Cytokeratin endo A antibody

• Cytokeratin-8 antibody

• DreK8 antibody

• EndoA antibody

• K2C8 antibody

• K2C8_HUMAN antibody

• K8 antibody

• Keratin 8 antibody

• Keratin type II cytoskeletal 8 antibody

• Keratin, type II cytoskeletal 8 antibody

• Keratin-8 antibody

• KO antibody

• Krt 2.8 antibody

• KRT8 antibody

• MGC118110 antibody

• MGC174782 antibody

• MGC53564 antibody

• MGC85764 antibody

• sb:cb186 antibody

• Type-II keratin Kb8 antibody

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)

  IHC image of ab2530 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2530, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (ab2530)

  ICC/IF image of ab2530 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2530, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

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  Western blot - Anti-Cytokeratin 8 antibody [C-43] (ab2530)

  Anti-Cytokeratin 8 antibody [C-43] (ab2530) at 2 µg/ml + HeLA cell lysate
  
  Predicted band size: 52.5 kDa
  
  

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  Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)

  Overlay histogram showing MCF7 cells stained with ab2530 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2530, 0.1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions. 

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