Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6]

• 产品名称

  Anti-Heparan Sulfate Proteoglycan 2/Perlecan抗体[A7L6]
  参阅全部 Heparan Sulfate Proteoglycan 2/Perlecan 一抗

• 描述

  大鼠单克隆抗体[A7L6] to Heparan Sulfate Proteoglycan 2/Perlecan

• 宿主

  Rat

• 特异性

  Recognizes domain IV of heparan sulphate proteoglycan 2 / perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment.

• 经测试应用

  适用于: ICC, IHC-P, IP, IHC-Fr, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human, Pig, Fish
  

• 免疫原

  Tissue, cells or virus corresponding to Heparan Sulfate Proteoglycan 2/Perlecan. High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
  Database link: P98160

• 常规说明

  Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.

  Source: A7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

  Formulation: The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 0.1% BSA

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• Primary antibody说明

  Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.

• 克隆

  单克隆

• 克隆编号

  A7L6

• 骨髓瘤

  x63-Ag8.653

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab2501于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 444872 Cow

• Entrez Gene: 3339 Human

• Entrez Gene: 15530 Mouse

• Entrez Gene: 313641 Rat

• Omim: 142461 Human

• SwissProt: P98160 Human

• SwissProt: Q05793 Mouse

• Unigene: 562227 Human

• Unigene: 273662 Mouse

别名

• Basement membrane specific heparan sulfate proteoglycan core protein antibody

• Endorepellin (domain V region) antibody

• Heparan sulfate proteoglycan of basement membrane antibody

• HSPG 2 antibody

• HSPG antibody

• Hspg2 antibody

• LG3 peptide antibody

• Perlecan antibody

• PGBM_HUMAN antibody

• PLC antibody

• Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody

• SJA antibody

• SJS antibody

• SJS1 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)

  IHC image on a frozen section of human colon showing strong reactivity in the extracellular matrix and basement membrane.

• 

  Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)

  Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.