Anti-MYL12A (phospho S19) antibody

• 产品名称

  Anti-MYL12A (phospho S19)抗体
  参阅全部 MYL12A 一抗

• 描述

  兔多克隆抗体to MYL12A (phospho S19)

• 宿主

  Rabbit

• 特异性

  ab2480 can detect the phosphorylated serine residue in both the human and mouse protein. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

  This phospho specific polyclonal antibody is specific for phosphorylated pS19 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

  Customers have seen variable performance with Xenopus laevis.

• 经测试应用

  适用于: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IPmore details

• 种属反应性

  与反应: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
  预测可用于: Bird, Mammals

• 免疫原

  Synthetic peptide corresponding to Human MYL12A aa 1-100 (phospho S19) conjugated to keyhole limpet haemocyanin.
  Database link: P19105

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 浓度

• 50 µg 浓度为 1.19 mg/ml

• 50 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 纯化说明

  This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2480于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 10627 Human

• Entrez Gene: 67268 Mouse

• Entrez Gene: 501203 Rat

• SwissProt: P19105 Human

• SwissProt: D3YV37 Mouse

• Unigene: 190086 Human

• 别名

• MLCB antibody

• MRCL3 antibody

• MYL12A antibody

• Myosin regulatory light chain 12A antibody

• Myosin regulatory light chain 2 antibody

• Myosin regulatory light chain 2, nonsarcomeric antibody

• Myosin regulatory light chain MRCL3 antibody

• Myosin regulatory light chain MRLC3 antibody

• Myosin RLC antibody

• Myosin, light polypeptide, regulatory, non sarcomeric (20kD) antibody

• nonsarcomeric antibody

• RLC antibody

• ML12A_HUMAN antibody

• MLC 2B antibody

• MLC-2B antibody

• 

  Western blot - Anti-MYL12A (phospho S19) antibody (ab2480)

  All lanes : Anti-MYL12A (phospho S19) antibody (ab2480) at 1/1000 dilution
  
  Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein, 50 ng
  Lane 2 : Regulatory Light Chain Phospho recombinant protein, 50 ng
  Lane 3 : Smooth Muscle Non-Phospho recombinant protein, 50 ng
  Lane 4 : Smooth Muscle Phospho recombinant protein, 50 ng
  
  Secondary
  All lanes : Peroxidase rabbit secondary antibody at 1/40000 dilution
  
  Predicted band size: 20 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12A (phospho S19) antibody (ab2480)

  IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

• 

  Western blot - Anti-MYL12A (phospho S19) antibody (ab2480)

  Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock-treated or CLA treated:
  Lane 1 : untreated 45 min
  Lane 2 : CLA 50 nm 45 min
  Lane 3 : CLA 100 nm 45 min
  Lane 4 : A21 untreated 45 min
  Lane 5 : A22 A23187 5 min
  Lane 6 : A23 A23187 15 min
  Lane 7 : A24 A23187 60 min
  After washing, a 1/5,000 dilution of anti-rabbit HRP (ab7090) was used as secondary.

  Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20