Anti-NF2 / Merlin (phospho S518) antibody

• 产品名称

  Anti-NF2 / Merlin (phospho S518)抗体
  参阅全部 NF2 / Merlin 一抗

• 描述

  兔多克隆抗体to NF2 / Merlin (phospho S518)

• 宿主

  Rabbit

• 特异性

  This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product). This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product).  Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA.  Cross-reactivity with NF2 (Merlin) occurs in mouse tissue.  Reactivity with NF2 from other sources has not been determined.

• 经测试应用

  适用于: IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide corresponding to Human NF2/ Merlin aa 500-600 (phospho S518) conjugated to keyhole limpet haemocyanin.

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product). The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2478于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4771 Human

• Entrez Gene: 18016 Mouse

• Omim: 607379 Human

• SwissProt: P35240 Human

• SwissProt: P46662 Mouse

• Unigene: 187898 Human

• Unigene: 297109 Mouse

• 别名

• MERL_HUMAN antibody

• Merlin antibody

• Moesin ezrin radixin like protein antibody

• Moesin ezrin radizin like antibody

• Moesin-ezrin-radixin-like protein antibody

• Neurofibromatosis 2 antibody

• Neurofibromatosis type 2 antibody

• Neurofibromatosis2 antibody

• Neurofibromin 2 antibody

• Neurofibromin-2 antibody

• Neurofibromin2 antibody

• NF 2 antibody

• Nf2 antibody

• SCH antibody

• Schwannomerlin antibody

• Schwannomin antibody

• ACN antibody

• BANF antibody

• Bilateral acoustic neuroma antibody

• 

  Western blot - Anti-NF2 / Merlin (phospho S518) antibody (ab2478)

  Rabbit polyclonal to phospho NF2 (Merlin) Ser 518 (ab2478) was used at a 1/1000 dilution to detect NF2 by Western blot. 

  Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2,3 and 4 respectively) for 45'.  After washing, a 1/5,000 dilution of HRP conjugated Goat-anti-Rabbit IgG (ab7090) was used as secondary antibody.

  Rabbit polyclonal to phospho NF2 (Merlin) Ser 518 (ab2478) was used at a 1/1000 dilution to detect NF2 by Western blot.
  
  Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2,3 and 4 respectively) for 45'. After washing, a 1/5,000 dilution of HRP conjugated Goat-anti-Rabbit IgG (ab7090) was used as secondary antibody.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin (phospho S518) antibody (ab2478)

  IHC image of ab2478 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.