Anti-NQO1抗体
参阅全部 NQO1 一抗
山羊多克隆抗体to NQO1
Goat
This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1)
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Rat, Human, Pig
预测可用于: Dog
Synthetic peptide corresponding to Human NQO1 aa 267-274 (C terminal).
Sequence:
C-SIPTDNQIKARK
(Peptide available as ab22904)
WB: U251, HepG2, Human Kidney, Human Lung, Human Duodenum, Rat Kidney, Rat Lung and Rat Duodenum lysates. IHC-P: Human Kidney tissue. ICC/IF: HepG2 cells.
Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab2346于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-32 kDa. Primary incubation for 1 hour at room temperature. | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 1728 Human
Omim: 125860 Human
SwissProt: P15559 Human
Unigene: 406515 Human
Unigene: 11234 Rat
Azoreductase antibody
Cytochrome b 5 reductase antibody
DHQU antibody
DIA 4 antibody
DIA4 antibody
Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
Diaphorase (NADH/NADPH) antibody
Diaphorase 4 antibody
Dioxin inducible 1 antibody
DT diaphorase antibody
DT-diaphorase antibody
DTD antibody
Menadione reductase antibody
NAD(P)H dehydrogenase [quinone] 1 antibody
NAD(P)H dehydrogenase quinone 1 antibody
NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
NAD(P)H quinone dehydrogenase 1 antibody
NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
NAD(P)H:menadione oxidoreductase 1 antibody
NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
NAD(P)H:quinone oxidoreductase 1 antibody
NAD(P)H:quinone oxireductase antibody
NMOR 1 antibody
NMOR I antibody
NMOR1 antibody
NMORI antibody
NQO 1 antibody
NQO1 antibody
NQO1_HUMAN antibody
Phylloquinone reductase antibody
QR 1 antibody
QR1 antibody
Quinone reductase 1 antibody
Western blot - Anti-NQO1 antibody (ab2346)
All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : Rat Kidney lysate in RIPA buffer
Lane 2 : Rat Lung lysate in RIPA buffer
Lane 3 : Rat Duodenum lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30-32 kDawhy is the actual band size different from the predicted?
Detected by chemiluminescence.
Western blot - Anti-NQO1 antibody (ab2346)
All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : Human Kidney lysate in RIPA buffer
Lane 2 : Human Lung lysate in RIPA buffer
Lane 3 : Human Duodenum lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30 kDawhy is the actual band size different from the predicted?
Detected by chemiluminescence.
Western blot - Anti-NQO1 antibody (ab2346)
All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : U251 cell lysate in RIPA buffer
Lane 2 : HepG2 cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30 kDawhy is the actual band size different from the predicted?
Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab2346)
ab2346 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)
ab2346 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
