Anti-Glutathione antibody [D8]

• 产品名称

  Anti-Glutathione抗体[D8]

• 描述

  小鼠单克隆抗体[D8] to Glutathione

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, ICC/IFmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Glutathione conjugated to KLH

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

• 存储溶液

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  D8

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab19534于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 别名

• Glutathione antibody

• GSH antibody

• Oxidised glutathione antibody

• Reduced glutathione antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534)Image courtesy of Dr Mahesh Shivananjappa by Abreview.

  ab19534 staining Glutathione in cultured murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.
  Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab19534 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/1000 dilution.

  See Abreview

• 

  Flow Cytometry - Anti-Glutathione antibody [D8] (ab19534)Image courtesy of Dr Mahesh Shivananjappa by Abreview.

  ab19534 at a 1/250 dilution detecting Glutathione in human monocytes by Flow Cytometry. An Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) secondary was used at a 1/500 dilution.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534)Image from Lim SY et al, J Biol Chem. 2010 May 7;285(19):14377-88. Epub 2010 Mar 11, Fig 4.

  ab19534 staining Glutathione in human neutrophils by Immunocytochemistry/ Immunofluorescence.
  Samples were fixed with 4% (w/v) paraformaldehyde in PBS, permeabilized with PBS containing 0.5% (w/v) saponin and 0.1% (w/v) bovine serum albumin, and then blocked with 1% (w/v) bovine serum albumin in PBS for 1 hour. ab19534 was used at 5µg/ml for 2 hours at room temperature. After washing with PBS, cells were incubated with Alexa 488-conjugated anti-mouse IgG at a 1/200 dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534)

  ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
  The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 µg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody.