Anti-Ctip1/BCL-11A antibody [18B12DE6]

• 产品名称

  Anti-Ctip1/BCL-11A抗体[18B12DE6]
  参阅全部 Ctip1/BCL-11A 一抗

• 描述

  小鼠单克隆抗体[18B12DE6] to Ctip1/BCL-11A

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WB, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Fusion protein corresponding to Human Ctip1/BCL-11A.
  Database link: Q9H165

• 表位

  Epitope is in carboxyl terminus of CTIP1/Bcl11a (aa's 434-776).

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  pH: 7.50
  Preservative: 0.02% Sodium azide
  Constituent: HEPES

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  IgG fraction

• 克隆

  单克隆

• 克隆编号

  18B12DE6

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab19489于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 53687 Mouse

• Unigene: 88824 Mouse

• Entrez Gene: 53335 Human

• Entrez Gene: 14025 Mouse

• Omim: 606557 Human

• SwissProt: Q9H165 Human

• SwissProt: Q9QYE3 Mouse

• Unigene: 370549 Human

• Unigene: 441102 Mouse

• Unigene: 471050 Mouse

• 别名

• B-cell CLL/lymphoma 11A antibody

• B-cell lymphoma/leukemia 11A antibody

• BC11A_HUMAN antibody

• BCL-11A antibody

• BCL11A antibody

• BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody

• BCL11A L antibody

• BCL11A S antibody

• BCL11A XL antibody

• BCL11a-M antibody

• BCL11AL antibody

• BCL11AS antibody

• BCL11AXL antibody

• C2H2 type zinc finger protein antibody

• COUP TF interacting protein 1 antibody

• COUP-TF-interacting protein 1 antibody

• CTIP1 antibody

• CTIP1, mouse, homolog of antibody

• D930021L15Rik antibody

• Ecotropic viral integration site 9 antibody

• Ecotropic viral integration site 9 homolog antibody

• Ecotropic viral integration site 9 protein antibody

• Ecotropic viral integration site 9 protein homolog antibody

• EVI-9 antibody

• Evi9 antibody

• Evi9, mouse, homolog of antibody

• FLJ10173 antibody

• FLJ34997 antibody

• HBFQTL5 antibody

• KIAA1809 antibody

• mKIAA1809 antibody

• OTTHUMP00000159788 antibody

• OTTHUMP00000159789 antibody

• OTTHUMP00000201250 antibody

• OTTHUMP00000202084 antibody

• Zinc finger protein 856 antibody

• ZNF856 antibody

• 2810047E18Rik antibody

• B cell CLL/lymphoma 11A (zinc finger protein) antibody

• B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody

• 

  Western blot - Anti-Ctip1/BCL-11A antibody [18B12DE6] (ab19489)

  Lane 1: Wild type HAP1 whole cell lysate (20 µg)
  Lane 2: Empty (0 µg)
  Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 µg)
  Lane 4: Raji whole cell lysate (20 µg)

  Lanes 1 - 4: Merged signal (red and green). Green - ab19489 observed at 91 kDa. Red - loading control, ab181602, observed at 37 kDa.

  ab19489 was shown to recognize BCL11A (Ctip1) in wild type cells as signal was lost at the expected MW in BCL11A (Ctip1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Empty knockout samples were subjected to SDS-PAGE. ab19489 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [18B12DE6] (ab19489)

  Human normal tonsil. Staining is localised to cytoplasm and nuclei. Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Flow Cytometry - Anti-Ctip1/BCL-11A antibody [18B12DE6] (ab19489)

  Overlay histogram showing Ramos cells stained with ab19489 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19489, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.