Anti-Ctip1/BCL-11A antibody [14B5]

• 产品名称

  Anti-Ctip1/BCL-11A抗体[14B5]
  参阅全部 Ctip1/BCL-11A 一抗

• 描述

  小鼠单克隆抗体[14B5] to Ctip1/BCL-11A

• 宿主

  Mouse

• 特异性

  Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for suitable positive controls.

• 经测试应用

  适用于: WB, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Fusion protein corresponding to Ctip1/BCL-11A.
  Database link: Q9H165

• 表位

  Epitope is in core of CTIP1/Bcl11a (aa's 172-434).

• 阳性对照

• WB: Raji whole cell lysate, Jurkat cell line, MEF cell line and HEK293T cell line. Flow Cyt: Ramos cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  pH: 7.50
  Preservative: 0.02% Sodium azide
  Constituent: HEPES

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Multi-step Chromatography

• 克隆

  单克隆

• 克隆编号

  14B5

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab19487于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 53687 Mouse

• Unigene: 88824 Mouse

• Entrez Gene: 53335 Human

• Entrez Gene: 14025 Mouse

• Omim: 606557 Human

• SwissProt: Q9H165 Human

• SwissProt: Q9QYE3 Mouse

• Unigene: 370549 Human

• Unigene: 441102 Mouse

• Unigene: 471050 Mouse

• 别名

• B-cell CLL/lymphoma 11A antibody

• B-cell lymphoma/leukemia 11A antibody

• BC11A_HUMAN antibody

• BCL-11A antibody

• BCL11A antibody

• BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody

• BCL11A L antibody

• BCL11A S antibody

• BCL11A XL antibody

• BCL11a-M antibody

• BCL11AL antibody

• BCL11AS antibody

• BCL11AXL antibody

• C2H2 type zinc finger protein antibody

• COUP TF interacting protein 1 antibody

• COUP-TF-interacting protein 1 antibody

• CTIP1 antibody

• CTIP1, mouse, homolog of antibody

• D930021L15Rik antibody

• Ecotropic viral integration site 9 antibody

• Ecotropic viral integration site 9 homolog antibody

• Ecotropic viral integration site 9 protein antibody

• Ecotropic viral integration site 9 protein homolog antibody

• EVI-9 antibody

• Evi9 antibody

• Evi9, mouse, homolog of antibody

• FLJ10173 antibody

• FLJ34997 antibody

• HBFQTL5 antibody

• KIAA1809 antibody

• mKIAA1809 antibody

• OTTHUMP00000159788 antibody

• OTTHUMP00000159789 antibody

• OTTHUMP00000201250 antibody

• OTTHUMP00000202084 antibody

• Zinc finger protein 856 antibody

• ZNF856 antibody

• 2810047E18Rik antibody

• B cell CLL/lymphoma 11A (zinc finger protein) antibody

• B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody

• 

  Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)

  Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
  Lane 2: Empty (0 µg)
  Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 µg)
  Lane 4: Raji whole cell lysate (20 µg)

  Lanes 1 - 4: Merged signal (red and green). Green - ab19487 observed at 91 kDa. Red - loading control, ab181602, observed at 37 kDa.

  ab19487 was shown to specifically react with BCL11A (Ctip1) in HAP1 wild type cells. No band was observed when BCL11A (Ctip1) knockout cells were examined. Wild-type and BCL11A (Ctip1) knockout samples were subjected to SDS-PAGE. ab19487 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

• 

  Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)

  All lanes : Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)
  
  Lane 1 : C6 Cell Line
  Lane 2 : MEF Cell Line
  Lane 3 : HDFn Cell LIne
  Lane 4 : Jurkat Cell Line
  Lane 5 : HEK293T Cell Line
  
  Lysates/proteins at 20 µg per lane.
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 91 kDa
  
  
  
  

  The CTIP1 / BCL-11A protein band is indicated at 92 kDa. This antibody also identified a 55 kDa protein band in Western blotting, principally in mouse fibroblasts (MEF), but this is unrelated to.

  Blocking was performed in 5% milk (in PBS). The primary antibody was diluted in 1% milk (in PBS) and incubated overnight at 4ºC.

• 

  Flow Cytometry - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)

  Overlay histogram showing Ramos cells stained with ab19487 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19487, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• 

  Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)

  Anti-Ctip1/BCL-11A antibody [14B5] (ab19487) at 1 µg/ml + Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 91 kDa
  Observed band size: 120 kDawhy is the actual band size different from the predicted?
  Additional bands at: 37 kDa, 42 kDa, 62 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 20 minutes
  
  
  

  The 120 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Ctip1 / BCL-11A.